Hair Regeneration Methods Using Cells Derived From Human Hair Follicles and Challenges to Overcome

    December 2024 in “ Cells ”
    Ons Ben Hamida, Moon Kyu Kim, Young Kwan Sung … Mi Hee Kwack
    Studysummary This study reviewed various methods and techniques aimed at overcoming limitations in culturing human hair follicles, specifically focusing on restoring the inductivity of hair follicle cells for successful hair regeneration.
    Automatically generated from the study's abstract, not written by a person, and not a review of the full paper. Not medical advice or a treatment recommendation. Read the original study, and consult a qualified healthcare professional before changing treatment. Full disclaimer
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    Research cited in this study 94

    1. Dermal Papilla Cells: From Basic Research to Translational Applications Biology · 2024
    2. Exosomes Derived From Mouse Vibrissa Dermal Papilla Cells Promote Hair Follicle Regeneration During Wound Healing By Activating Wnt/β-Catenin Signaling Pathway Journal of Nanobiotechnology · 2024
    3. A Review of Exosomes in Regenerative Cosmetic Dermatology Molecular frontiers journal · 2023
    4. Adipose-Derived Stem Cell Exosomes Antagonize the Inhibitory Effect of Dihydrotestosterone on Hair Follicle Growth by Activating Wnt/Beta-Catenin Pathway Stem Cells International · 2023
    5. In Vitro Induction of Hair Follicle Signatures Using Human Dermal Papilla Cells Encapsulated in Fibrin Microgels Cell Proliferation · 2023
    6. IPSC-Based Approach for Human Hair Follicle Regeneration Frontiers in Cell and Developmental Biology · 2023
    7. Hair Growth Promoting Effects of Human Dermal Papilla Cells in Pigs Experimental Dermatology · 2023
    8. Sox2 in the Dermal Papilla Regulates Hair Follicle Pigmentation Cell reports · 2022
    9. Characterization of the Epidermal-Dermal Junction in hiPSC-Derived Skin Organoids Stem cell reports · 2022
    10. Generation And Characterization Of Hair-Bearing Skin Organoids From Human Pluripotent Stem Cells Nature Protocols · 2022
    11. Effects of Exosomes from Adipose-Derived Stem Cells on Hair Loss: A Retrospective Analysis of 39 Patients Journal of cosmetic dermatology · 2022
    12. KY19382, A Novel Activator of Wnt/β-Catenin Signalling, Promotes Hair Regrowth and Hair Follicle Neogenesis British Journal of Pharmacology · 2021
    13. Functional Hair Follicle Regeneration: An Updated Review Signal Transduction and Targeted Therapy · 2021
    14. Neural Progenitor Cell-Derived Nanovesicles Promote Hair Follicle Growth via miR-100 Journal of nanobiotechnology · 2021
    15. Strategies to Utilize iPS Cells for Hair Follicle Regeneration and the Treatment of Hair Loss Disorders Elsevier eBooks · 2021
    16. Microscopy-Guided Laser Ablation for the Creation of Complex Skin Models with Folliculoid Appendages Bioengineering & translational medicine · 2020
    17. Hair-Bearing Human Skin Generated Entirely From Pluripotent Stem Cells Nature · 2020
    18. Current Advances in Stem Cell-Based Therapies for Hair Regeneration European Journal of Pharmacology · 2020
    19. Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold Biomolecules · 2020
    20. Reconstructed Human Skin Shows Epidermal Invagination Towards Integrated Neopapillae Indicating Early Hair Follicle Formation In Vitro Journal of Tissue Engineering and Regenerative Medicine · 2020
    21. Preliminary Studies of Hair Follicle Regeneration by Injections of Epidermal Stem Cells and Dermal Papilla Cells into Nude Mice Cell and Tissue Banking · 2020
    22. Autologous Cell-Based Therapy for Male and Female Pattern Hair Loss Using Dermal Sheath Cup Cells: A Randomized Placebo-Controlled Double-Blinded Dose-Finding Clinical Study Journal of The American Academy of Dermatology · 2020
    23. Cellular Nanofiber Structure with Secretory Activity-Promoting Characteristics for Multicellular Spheroid Formation and Hair Follicle Regeneration ACS Applied Materials & Interfaces · 2020
    24. Sustained Release of Dermal Papilla-Derived Extracellular Vesicles from Injectable Microgel Promotes Hair Growth Theranostics · 2020
    25. Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion Methods in molecular biology · 2020
    26. Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods Skin Pharmacology and Physiology · 2020
    27. Pre-Aggregation of Scalp Progenitor Dermal and Epidermal Stem Cells Activates the WNT Pathway and Promotes Hair Follicle Formation In Vitro and In Vivo Systems Stem Cell Research & Therapy · 2019
    28. Overexpression of Alkaline Phosphatase Improves the Hair-Inductive Capacity of Cultured Human Dermal Papilla Spheres Journal of Dermatological Science · 2019
    29. Characterization of Human Dermal Sheath Cells Reveals CD36-Expressing Perivascular Cells Associated with Capillary Blood Vessel Formation in Hair Follicles Biochemical and Biophysical Research Communications · 2019
    30. Wnt Activator CHIR99021-Stimulated Human Dermal Papilla Spheroids Contribute to Hair Follicle Formation and Production of Reconstituted Follicle-Enriched Human Skin Biochemical and Biophysical Research Communications · 2019
    31. Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development Cells · 2019
    32. A Therapeutic Microneedle Patch Made from Hair-Derived Keratin for Promoting Hair Regrowth ACS nano · 2019
    33. Exosomes Derived from Human Dermal Papilla Cells Promote Hair Growth in Cultured Human Hair Follicles and Augment the Hair-Inductive Capacity of Cultured Dermal Papilla Spheres Experimental Dermatology · 2019
    34. Injury Modifies the Fate of Hair Follicle Dermal Stem Cell Progeny in a Hair Cycle-Dependent Manner Experimental dermatology · 2019
    35. Use of Human Intra-Tissue Stem/Progenitor Cells and Induced Pluripotent Stem Cells for Hair Follicle Regeneration PubMed · 2019
    36. Conditioned Media Derived from Mesenchymal Stem Cell Cultures: The Next Generation for Regenerative Medicine Journal of Tissue Engineering and Regenerative Medicine · 2019
    37. Tissue Engineering of Human Hair Follicles Using a Biomimetic Developmental Approach Nature Communications · 2018
    38. Establishment of an In Vitro Organoid Model of Dermal Papilla of Human Hair Follicle Journal of Cellular Physiology · 2018
    39. A Bald Statement: Current Approaches to Manipulate Miniaturization Focus Only on Promoting Hair Growth Experimental Dermatology · 2018
    40. Regulation of Hair Follicle Development by Exosomes Derived from Dermal Papilla Cells Biochemical and Biophysical Research Communications · 2018
    41. Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids Cell Reports · 2018
    42. Skin Wound Healing in Humans and Mice: Challenges in Translational Research Journal of Dermatological Science · 2017
    43. Extracellular Vesicles Derived from MSCs Activate Dermal Papilla Cells In Vitro and Promote Hair Follicle Conversion from Telogen to Anagen in Mice Scientific Reports · 2017
    44. The Basic Mechanism of Hair Growth Stimulation by Adipose-Derived Stem Cells and Their Secretory Factors Current Stem Cell Research & Therapy · 2017
    45. Preservation of a Specialized Phenotype of Dermal Papilla Cells of a Human Hair Follicle Under Cultivation Conditions Biology bulletin/Biology bulletin of the Russian Academy of Sciences · 2017
    46. Stem Cells from Human Hair Follicles: First Mechanical Isolation for Immediate Autologous Clinical Use in Androgenetic Alopecia and Hair Loss Stem cell investigation · 2017
    47. As a Carrier–Transporter for Hair Follicle Reconstitution, Platelet-Rich Plasma Promotes Proliferation and Induction of Mouse Dermal Papilla Cells Scientific Reports · 2017
    48. Methods for the Isolation and 3D Culture of Dermal Papilla Cells from Human Hair Follicles Experimental Dermatology · 2017
    49. Induction of Hair Follicle Dermal Papilla Cell Properties in Human Induced Pluripotent Stem Cell-Derived Multipotent LNGFR(+)THY-1(+) Mesenchymal Cells Scientific Reports · 2017
    50. Induced Pluripotent Stem Cells From Human Hair Follicle Keratinocytes As A Potential Source For In Vitro Hair Follicle Cloning PeerJ · 2016
    51. Activin A-Induced Signaling Controls Hair Follicle Neogenesis Experimental Dermatology · 2016
    52. CD133-Positive Dermal Papilla-Derived Wnt Ligands Regulate Postnatal Hair Growth Biochemical Journal · 2016
    53. Hair Follicle and Sebaceous Gland De Novo Regeneration With Cultured Epidermal Stem Cells and Skin-Derived Precursors Stem Cells Translational Medicine · 2016
    54. Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay Methods in molecular biology · 2016
    55. Wnt1a Maintains Characteristics of Dermal Papilla Cells That Induce Mouse Hair Regeneration in a 3D Preculture System Journal of Tissue Engineering and Regenerative Medicine · 2015
    56. Hair Follicle Dermal Stem Cells Regenerate the Dermal Sheath, Repopulate the Dermal Papilla, and Modulate Hair Type Developmental Cell · 2014
    57. Controllable Production of Transplantable Adult Human High-Passage Dermal Papilla Spheroids Using 3D Matrigel Culture Tissue engineering. Part A · 2014
    58. Alopecia Areata Is Driven by Cytotoxic T Lymphocytes and Is Reversed by JAK Inhibition Nature medicine · 2014
    59. Human Epithelial Hair Follicle Stem Cells and Their Progeny: Current State of Knowledge, the Widening Gap in Translational Research and Future Challenges BioEssays · 2014
    60. Generation of Folliculogenic Human Epithelial Stem Cells from Induced Pluripotent Stem Cells Nature communications · 2014
    61. Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth Proceedings of the National Academy of Sciences of the United States of America · 2013
    62. Neonatal Murine Skin-Derived Cells Transplanted Using a Mini-Chamber Model Produce Robust and Normal Hair Journal of Tissue Engineering and Regenerative Medicine · 2013
    63. Human Induced Pluripotent Stem Cell-Derived Ectodermal Precursor Cells Contribute to Hair Follicle Morphogenesis In Vivo ˜The œjournal of investigative dermatology/Journal of investigative dermatology · 2013
    64. Scalable Production of Controllable Dermal Papilla Spheroids on PVA Surfaces and the Effects of Spheroid Size on Hair Follicle Regeneration Biomaterials · 2012
    65. Morphogenesis of Chimeric Hair Follicles in Engineered Skin Substitutes With Human Keratinocytes and Murine Dermal Papilla Cells Experimental Dermatology · 2012
    66. Conditioned Media Obtained from Human Outer Root Sheath Follicular Keratinocyte Culture Activates Signalling Pathways That Contribute to Maintenance of Hair-Inducing Capacity and Increases Trichogenicity of Cultured Dermal Cells Experimental Dermatology · 2012
    67. Hair Organ Regeneration Via Bioengineered Hair Follicular Unit Transplantation Scientific Reports · 2012
    68. Fully Functional Hair Follicle Regeneration Through the Rearrangement of Stem Cells and Their Niches Nature Communications · 2012
    69. Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay Journal of Investigative Dermatology · 2011
    70. Bald Scalp in Men with Androgenetic Alopecia Retains Hair Follicle Stem Cells but Lacks CD200-Rich and CD34-Positive Hair Follicle Progenitor Cells Journal of Clinical Investigation · 2011
    71. Hair Growth Stimulated by Conditioned Medium of Adipose-Derived Stem Cells Is Enhanced by Hypoxia: Evidence of Increased Growth Factor Secretion Biomedical research · 2010
    72. Review of Hair Follicle Dermal Cells Journal of Dermatological Science · 2009
    73. Hair Follicle Neogenesis Induced by Cultured Human Scalp Dermal Papilla Cells Regenerative Medicine · 2009
    74. Sox2-Positive Dermal Papilla Cells Specify Hair Follicle Type in Mammalian Epidermis Development · 2009
    75. TGF-β2 Is Specifically Expressed in Human Dermal Papilla Cells and Modulates Hair Folliculogenesis Journal of Cellular and Molecular Medicine · 2009
    76. Self-Assembly of Dermal Papilla Cells into Inductive Spheroidal Microtissues on Poly(Ethylene-Co-Vinyl Alcohol) Membranes for Hair Follicle Regeneration Biomaterials · 2008
    77. Isolation and Short-Term Culture of Primary Keratinocytes, Hair Follicle Populations, and Dermal Cells from Newborn Mice and Keratinocytes from Adult Mice for In Vitro Analysis and Grafting to Immunodeficient Mice Nature Protocols · 2008
    78. Hair Follicle Regeneration Using Grafted Rodent and Human Cells Journal of Investigative Dermatology · 2007
    79. Biological Characterization of Cultured Dermal Papilla Cells and Hair Follicle Regeneration In Vitro and In Vivo Chinese Medical Journal · 2006
    80. Characterization and Isolation of Stem Cell-Enriched Human Hair Follicle Bulge Cells ˜The œJournal of clinical investigation/˜The œjournal of clinical investigation · 2005
    81. Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle PLoS biology · 2005
    82. Hair Cycle-Specific Expression of Versican in Human Hair Follicles Journal of Dermatological Science · 2005
    83. Follicular Cell Implantation: An Emerging Cell Therapy for Hair Loss Seminars in Plastic Surgery · 2005
    84. Organogenesis From Dissociated Cells: Generation of Mature Cycling Hair Follicles From Skin-Derived Cells Journal of Investigative Dermatology · 2005
    85. Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla Journal of Investigative Dermatology · 2003
    86. Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34 Journal of Investigative Dermatology · 2003
    87. Morphogenesis and Renewal of Hair Follicles from Adult Multipotent Stem Cells Cell · 2001
    88. Involvement of Follicular Stem Cells in Forming Not Only the Follicle but Also the Epidermis Cell · 2000
    89. Multiple Roles for Activated LEF/TCF Transcription Complexes During Hair Follicle Development and Differentiation Development · 1999
    90. Dermal-Epidermal Interactions in Hair Follicle Development and Maintenance Dermatologic clinics · 1996
    91. Hair Matrix Germinative Epidermal Cells Confer Follicle-Inducing Capabilities on Dermal Sheath and High Passage Papilla Cells Development · 1996
    92. Dermal-Epidermal Interactions: Follicle-Derived Cell Populations in the Study of Hair-Growth Mechanisms ˜The œjournal of investigative dermatology/Journal of investigative dermatology · 1993
    93. The Secret Life of the Hair Follicle Trends in genetics · 1992
    94. Dermal-Epidermal Interactions in Hair Follicle Development and Cycling Clinics in dermatology · 1988

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    1. Regeneration of Hair Follicles Is Modulated by Flightless I (Flii) in a Rodent Vibrissa Model Journal of Investigative Dermatology · 2010