Preservation of a Specialized Phenotype of Dermal Papilla Cells of a Human Hair Follicle Under Cultivation Conditions

    Ekaterina P. Kalabusheva, E. S. Chermnykh, В. В. Терских, E. A. Vorotelyak
    Studysummary This study found that cultivating dermal papilla cells in spheroids or with valproic acid most effectively preserves their phenotype in vitro, compared to a monolayer culture with BMP6 and vitamin D3, which only produces a short-term effect.
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    Research cited in this study 19

    1. Assembling Composite Dermal Papilla Spheres with Adipose-Derived Stem Cells to Enhance Hair Follicle Induction Scientific Reports · 2016
    2. Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroids of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration ACS Applied Materials & Interfaces · 2016
    3. Pharmacologic Inhibition of JAK-STAT Signaling Promotes Hair Growth Science Advances · 2015
    4. Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth Proceedings of the National Academy of Sciences of the United States of America · 2013
    5. Valproic Acid Promotes Human Hair Growth in In Vitro Culture Model Journal of Dermatological Science · 2013
    6. Maintenance of High Proliferation and Multipotent Potential of Human Hair Follicle-Derived Mesenchymal Stem Cells by Growth Factors International Journal of Molecular Medicine · 2013
    7. Therapeutic Strategy for Hair Regeneration: Hair Cycle Activation, Niche Environment Modulation, Wound-Induced Follicle Neogenesis, and Stem Cell Engineering Expert Opinion on Biological Therapy · 2013
    8. Scalable Production of Controllable Dermal Papilla Spheroids on PVA Surfaces and the Effects of Spheroid Size on Hair Follicle Regeneration Biomaterials · 2012
    9. Mesenchymal–Epithelial Interactions During Hair Follicle Morphogenesis and Cycling Seminars in Cell & Developmental Biology · 2012
    10. Hairy Tale of Signaling in Hair Follicle Development and Cycling Seminars in cell & developmental biology · 2012
    11. 1α,25-Dihydroxyvitamin D3 Modulates the Hair-Inductive Capacity of Dermal Papilla Cells: Therapeutic Potential for Hair Regeneration Stem Cells Translational Medicine · 2012
    12. Valproic Acid Induces Hair Regeneration in Murine Model and Activates Alkaline Phosphatase Activity in Human Dermal Papilla Cells PLOS ONE · 2012
    13. Dermal Papilla Cells Induce Keratinocyte Tubulogenesis in Culture Histochemistry and cell biology · 2010
    14. The Enhancement of Dermal Papilla Cell Aggregation by Extracellular Matrix Proteins Through Effects on Cell–Substratum Adhesivity and Cell Motility Biomaterials · 2009
    15. TGF-β2 Is Specifically Expressed in Human Dermal Papilla Cells and Modulates Hair Folliculogenesis Journal of Cellular and Molecular Medicine · 2009
    16. The Hair Follicle as a Dynamic Mini-Organ Current Biology · 2009
    17. Physical And Functional Interaction Between The Vitamin D Receptor And Hairless Corepressor, Two Proteins Required For Hair Cycling Journal of Biological Chemistry · 2003
    18. Molecular Biology of Hair Morphogenesis: Development and Cycling Journal Of Experimental Zoology Part B: Molecular And Developmental Evolution · 2003
    19. Effects of Epidermal Growth Factor, Fibroblast Growth Factor, Minoxidil, and Hydrocortisone on Growth Kinetics in Human Hair Bulb Papilla Cells and Root Sheath Fibroblasts Cultured In Vitro Archives of Dermatological Research · 1987

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