Maintenance of High Proliferation and Multipotent Potential of Human Hair Follicle-Derived Mesenchymal Stem Cells by Growth Factors
February 2013
in “
International Journal of Molecular Medicine
”
human hair follicle-derived mesenchymal stem cells growth factors basic fibroblast growth factor acidic fibroblast growth factor epidermal growth factor self-renewal high proliferation multipotent differentiation adipocytes chondrocytes osteoblasts proliferating cell nuclear antigen HF-MSCs bFGF aFGF EGF PCNA
TLDR Growth factors help hair follicle stem cells grow and stay versatile.
The study investigated the effects of growth factors on human hair follicle-derived mesenchymal stem cells (HF-MSCs), revealing that basic fibroblast growth factor (bFGF), acidic fibroblast growth factor (aFGF), and epidermal growth factor (EGF) significantly enhanced their proliferation and maintained their multipotent potential. HF-MSCs could differentiate into adipocytes, chondrocytes, and osteoblasts, similar to bone marrow mesenchymal stem cells. The study found that bFGF and EGF were effective at concentrations as low as 1 ng/ml, while aFGF required concentrations above 5 ng/ml. More than 95% of HF-MSCs cultured with these growth factors showed high proliferation, and they retained their trilineage differentiation potential, unlike those cultured without growth factors. The findings suggested that HF-MSCs could be a viable source for tissue engineering and regenerative medicine.
