Cells, Growth Factors and Biomaterials Used in Tissue Engineering for Hair Follicles Regeneration

This study found that GelMA/HAMA bioink shows promise for 3D printing skin equivalents, as it effectively mimics the native skin's properties and supports hair follicle structure development.

This study found that activating the PI3K/Akt signaling pathway in dermal papilla cells enhances their hair-inducing capabilities, suggesting potential strategies for hair regenerative medicine.

This study summarizes the signaling molecules and extracellular components involved in epithelial–mesenchymal interactions, enhancing our understanding of hair follicle regeneration and skin wound healing, but reports no new experimental results.

This article reviews current knowledge and research gaps on growth hormone in hair follicle biology, highlighting its complex role and suggesting further exploration to reveal nonclassical skin functions.

In this study, structural and functional brain abnormalities specific to visuospatial and somatosensory processing networks were observed in patients with anorexia nervosa, highlighting a critical role for the precuneus.

This study found that TGF-β2 may enhance collagen expression and spheroid formation in human dermal papilla cells, which could improve their hair inductivity and maintenance.

This review examines the life cycle, biomarkers, and functions of hair follicle stem cells, emphasizing their role in hair loss therapy and other skin and hair disorders, but reports no new clinical results.

This review examines the challenges of hair follicle regeneration and outlines strategies for bioengineering human hair follicle models, without presenting new experimental results.

This review explains the evolution and current techniques of follicular unit transplantation for hair restoration, identifying conditions suitable for surgery and offering guidelines for dermatologists to assess candidate suitability, focusing on male and female pattern hair loss.

This review evaluates in vitro hair follicle models, techniques, and challenges without reporting new clinical results, emphasizing their future potential in hair transplantation.

This review discusses human hair follicle development and highlights the potential of creating human in vitro models and skin substitutes with hair follicles, reporting no new clinical results.

This study demonstrated that adding human adipose-derived stem cells to hair follicle germ-like aggregates significantly improved hair shaft generation in transplanted nude mice, suggesting a promising strategy for hair regenerative medicine.

This review discusses potential cell sources and bioengineering strategies for regenerating hair follicles with functional cycling, reporting no new results.

This review found that platelet-rich plasma therapy improved hair growth and thickness in most studies on alopecia, with the highest improvement seen when combined with Minoxidil.

In this study, hair follicle germs containing vascular endothelial cells showed improved hair regeneration and morphogenesis-related gene expression in mice, suggesting a promising strategy for hair regenerative medicine.

This review explores recent insights into how intrinsic gene oscillations and molecular interactions in hair follicle stem cells contribute to their regenerative potential, with potential implications for regenerative medicine, but reports no new clinical results.

This article reviews the classification, diagnosis, and management of hair disorders, emphasizing the role of trichoscopy and early diagnosis for effective treatment but reports no new clinical findings.

This study reports that using activated platelet-rich plasma releasate in the preparation of bioengineered hair follicle germs enhanced follicular gene expression and significantly improved hair regeneration in vitro.

In this study, EREG promoted hair growth by activating specific receptors in cell and animal models, indicating it may be a potential treatment for hair loss.

This study concluded that oral minoxidil is an effective and well-tolerated alternative to topical minoxidil for healthy patients struggling with application-related compliance issues.

This study demonstrates that incorporating human neopapilla into reconstructed human skin can initiate hair follicle morphogenesis by stimulating epidermal invagination and maintaining follicle-inductive properties in vitro.

This study found that injecting a mixture of epidermal stem cells and dermal papilla cells can induce hair follicle regeneration and well-ordered epidermis formation in mice.

This study reported that a novel chitosan/polyvinyl alcohol nanofiber sponge effectively enhances the hair follicle-inducing ability of dermal papilla multicellular spheroids in a mouse model.

This study found that BMP2 increased PTEN expression and induced autophagy, promoting hair follicle stem cell differentiation in both in vitro models and a mouse wound model.

This study found that the human placenta extracellular matrix hydrogel restored the hair-inductive capacity of high-passaged dermal papilla cells, enabling them to regenerate new hair follicles when co-grafted with mouse epidermal cells.

In this study, researchers developed a chemical cocktail and suspension culture method to transform human dermal fibroblasts into dermal papilla-like cells capable of inducing hair follicle formation in vitro and in vivo.

This study developed a method for generating germ-like tissues called bbHFGs, which efficiently promoted hair follicle formation in mice, potentially advancing hair regenerative medicine applications.

This study evaluated the effectiveness and safety of low-dose oral minoxidil (2.5-5 mg daily) in men with male androgenetic alopecia, aiming to contribute data to an area with limited previous reports. Results are not provided in the abstract.

In this study, growing human hair follicles in a defined glycosaminoglycan hydrogel ex vivo promoted cell survival, proliferation, and activation of hair growth-related signaling pathways, suggesting GAG-based hydrogels could modulate the hair follicle growth cycle.

This study found that TSA restored the hair-inductive capacity of skin-derived precursors in mice by enhancing BMP gene expression and signaling through histone acetylation.

This study found that BMP6 and Wnt10b competitively regulate the transition between hair follicle growth phases, offering new insights into hair follicle cycling and potential hair loss treatments.

This study demonstrated that enhancing human skin constructs with hair follicles through engineered cell organization and vascularization improved hair growth in immunodeficient mice, suggesting potential advances for treating alopecia and chronic wounds.

This review discusses the classification and diagnosis of primary cicatricial alopecias and reports no new clinical results; it highlights the need for effective treatments owing to limited evidence of current therapies' efficacy.

This study reported that once-daily treatment with oral minoxidil and spironolactone for 12 months reduced hair loss severity and hair shedding in women with pattern hair loss, with generally mild side effects, though results came from an uncontrolled, observational pilot study.

This study reports that using nanogel and layer-by-layer self-assembly to encapsulate single dermal papilla cells can form cell spheroids that regenerate hair follicles successfully in a hair follicle regeneration model.

This study demonstrated a method for large-scale preparation of self-sorted hair follicle germs in vitro, which successfully generated hair follicles when transplanted into the skin of nude mice.

This review examines controversies in hair transplant surgery, including donor harvesting techniques and the roles of platelet-rich plasma and low-level light therapy, and reports no new clinical results.

This article reviews different types of alopecia, their diagnosis, and treatment options available in a family physician's practice, but it reports no new research findings.

This review synthesizes recent findings on the hair follicle stem cell niche and its complex interactions, exploring potential pathways for developing new hair growth therapies; it reports no new clinical results.

This article details laboratory methods for isolating and culturing human dermal papilla cells but provides no new experimental results.

In this study, researchers differentiated induced pluripotent stem cells into cells with dermal papilla-like properties, demonstrating their potential role in hair follicle bioengineering and drug testing for hair growth.

This study found that while extracellular matrix components like hyaluronic acid promoted larger organoid formation, other matrix components hindered hair follicle germ assembly in an in vitro model.

This study established a new in vitro culture system that supports the growth and dynamic self-organization of hair follicle stem cells and their progeny, promoting a stable population equilibrium.

This study found that platelet-rich plasma may influence the proliferation of human hair dermal papilla cells by affecting gene expression related to the cell cycle.

In this study, using stirred-suspension bioreactors to expand dermal stem cells resulted in larger numbers, but had reduced efficiency in forming hair follicle structures compared to static culture conditions.

This study found that Wnt ligands from CD133-positive dermal papilla cells are essential for maintaining hair growth by supporting cell signaling between different hair follicle structures.

This study demonstrated that cultured epidermal stem cells and skin-derived precursors can regenerate functional hair follicles and sebaceous glands in both mice and adult humans, suggesting potential for bioengineered skin substitutes.

The study found that up-regulating β-catenin signaling in CD 133-positive dermal papilla cells enhanced their ability to promote hair follicle regeneration both in vitro and in vivo.

In this study, the RADA-PRG hydrogel enhanced SKP proliferation, survival, and gene expression, effectively supporting hair follicle regeneration in mice.

This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.

This research found that Foxc1 transcription factor and COL17A1 are critical in regulating quiescence and hair thinning in hair follicle stem cells, with aging-related DNA damage leading to hair loss.

This study observed that applying JAK-STAT pathway inhibitors to mouse and human skin led to rapid hair growth by inducing the anagen phase of hair follicles.

This article presents a comprehensive guide for classifying human hair follicle cycle stages in vivo using scalp xenografts on immunocompromised mice, offering valuable resources for researchers in the field.

This study demonstrated that a 3D air–liquid culture system using Wnt-CM-treated cells can induce hair regeneration in nude mice and may facilitate large-scale preparation for hair loss treatment.

Fibroblasts can be turned into melanocytes for potential skin treatments.

This study found that applying 10% platelet-rich plasma significantly increased hair follicle density and reduced the time for hair formation in a mouse model compared to no PRP treatment.

This study found that ectodermal precursor cells derived from human induced pluripotent stem cells may enhance hair follicle morphogenesis through improved epithelial-mesenchymal interactions when cocultured with dermal cells.

This study found that dermal fibroblast cultures, especially those with higher alpha-smooth muscle actin expression, can be used to produce skin-derived precursor cells, unlike human hair follicle dermal papilla cultures.

This review discusses the biology of hair follicle stem cells and highlights their potential for applications in regenerative medicine, drug, and gene delivery, but reports no new clinical results.

This study found that both human and rat dermal papilla spheroids can induce hair follicle neogenesis, but larger spheroids increase inductivity without significantly affecting hair fiber diameter.

In this study, VD(3) pretreatment increased hair follicle formation in a rat model by promoting dermal papilla cell differentiation, suggesting potential for hair regeneration therapy.

This study demonstrated that bioengineered hair follicles reconstituted from embryonic skin cells and transplanted into hosts can restore physiological hair functions, suggesting potential applications for treating alopecia.

This study demonstrates the potential of using bioengineered follicles from adult tissue-derived stem cells for fully functional hair regeneration, illustrating possible applications in organ replacement therapy.

This study identified TGF-β2 as a crucial signal in hair follicle stem cell regeneration, highlighting its role in counteracting BMP signaling to promote tissue regeneration.

This study found that adult mouse dermis can be reprogrammed by epidermal cues to resemble neonatal dermis, with changes in fibroblast proliferation and extracellular matrix composition.

This study found that cultured human dermal papilla cells formed into three-dimensional spheres have an increased ability to induce hair follicles from mouse epidermal cells compared to two-dimensional cultures.

This study found that human hair follicle-derived mesenchymal stem cells can differentiate into myogenic, osteogenic, adipogenic, and chondrogenic lineages, although this potential decreases over time and varies by lineage.

This study found that TSC2-null fibroblast cells from TSC skin hamartomas can induce hair follicle formation and hamartomatous changes in keratinocytes, with active mTOR signaling observable in a mouse xenograft model.

COL17A1 is crucial for preventing hair graying and loss by supporting hair and pigment stem cells.

This study reveals that stem cell descendants in mouse hair follicles can return to their niche to retain stemness and function as primary stem cells for future hair cycles.

This study suggests that skin-derived precursors from Sox2(+) hair follicle dermal cells have the potential to function as dermal stem cells, supporting hair morphogenesis, dermal maintenance, and wound-healing.

This review summarizes fundamental concepts and recent advancements in hair follicle biology, including insights from mouse models into broader molecular and cellular processes relevant to regeneration and development.

Lgr5 is a marker for active, long-lasting stem cells in mouse hair follicles.

This study found that laminin-511 is crucial for hair morphogenesis in mice, as it influences primary cilia formation and dermal papilla maintenance through noggin and sonic hedgehog signaling.

This study found that Myc activation in epidermal stem cells leads to chromatin modifications that promote stem cell exit and differentiation, which can be influenced by HDAC inhibitors like trichostatin A.

Hair follicles can regrow in wounded adult mouse skin using a process like embryo development.

This study found that bulge cells marked by CD200+ in human hair follicles show high colony-forming efficiency, suggesting successful enrichment of keratinocyte stem cells.

This study developed molecular signatures for dermal papilla cells and their niche, uncovering novel signaling regulators and genes linked to hair disorders, which may inform future hair development research.

This review examines the role of neurotrophins in hair follicle development and remodeling, highlighting potential therapeutic applications for hair growth disorders, but it reports no new empirical findings.

This study demonstrated that clonal dermal papilla and dermal sheath cell lines from hair follicles can differentiate into lipid and bone cells, suggesting their potential as a stem cell source for tissue engineering.

This review summarizes recent advances in understanding the molecular mechanisms of hair follicle formation and discusses potential future clinical applications for treating hair loss and skin tumors, but it reports no new clinical results.

In this study, researchers found that p75 neurotrophin receptor signaling affects apoptosis during hair follicle regression in mice, and altering its activity might help manage disorders involving early catagen entry.

This study found that LEF1/TCF3 is necessary but not sufficient for TOPGAL activation in hair follicle development, indicating complex regulation in the skin.

This article discusses the distribution of extracellular matrix molecules in human hair follicles and reports no new results.