TGF-β2 and Collagen Play Pivotal Roles in the Spheroid Formation and Anti-Aging of Human Dermal Papilla Cells

August 2021 in “ Aging ”

Hyun-Ju Kim, Nahyun Choi, Doo Yeong Kim, So Yoon Kim, Seung Yong Song, Jong‐Hyuk Sung

Transforming Growth Factor-beta 2 TGF-β2 collagen human dermal papilla cells hDPCs cell spheroids anti-aging genes cellular senescence COL13A1 COL15A1 3D-cultured hDPCs hair growth spheroid formation dermal papilla cells anti-aging cell aging

TLDR Collagen and TGF-β2 help maintain hair cell shape and youthfulness.

The study found that Transforming Growth Factor-beta 2 (TGF-β2) and collagen significantly contribute to the formation of cell spheroids and have anti-aging effects on human dermal papilla cells (hDPCs), which are crucial for hair growth. TGF-β2 and collagen enhanced the expression of anti-aging genes, reduced cellular senescence, and promoted hair growth. The expression of collagens COL13A1 and COL15A1 was upregulated in 3D-cultured hDPCs, and this expression decreased with aging. TGF-β2 expression also decreases with aging and is involved in regulating the expression of COL13A1 and COL15A1. Supplementing with TGF-β2 delayed cellular senescence and recovered spheroid formation in aged hDPCs by upregulating collagen levels. Conversely, knocking out TGF-β2 induced the aging of DPCs and inhibited spheroid formation. TGF-β2 treatment also promoted hair growth in mice. The study suggests that TGF-β2 and collagen could potentially be used in treatments for hair loss and aging skin.

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