Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroids of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration

    February 2016 in “ ACS Applied Materials & Interfaces ”
    Bojie Lin, Yong Miao, Jin Wang … Malcolm Xing
    Studysummary This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.
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    Research cited in this study 29

    1. Extensive Hair-Shaft Elongation by Isolated Mouse Whisker Follicles in Very Long-Term Gelfoam Histoculture PLoS ONE · 2015
    2. A Simple and Rapid Model for Hair-Follicle Regeneration in the Nude Mouse Clinical and Experimental Dermatology · 2015
    3. Controllable Production of Transplantable Adult Human High-Passage Dermal Papilla Spheroids Using 3D Matrigel Culture Tissue engineering. Part A · 2014
    4. Early Inductive Events in Ectodermal Appendage Morphogenesis Seminars in cell & developmental biology · 2014
    5. Regenerative Medicine and Hair Loss: How Hair Follicle Culture Has Advanced Our Understanding of Treatment Options for Androgenetic Alopecia Regenerative Medicine · 2013
    6. Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth Proceedings of the National Academy of Sciences of the United States of America · 2013
    7. Promotional Effect of Platelet-Rich Plasma on Hair Follicle Reconstitution In Vivo Dermatologic Surgery · 2013
    8. Future Horizons in Hair Restoration Facial Plastic Surgery Clinics of North America · 2013
    9. Follicular Dermal Papilla Structures by Organization of Epithelial and Mesenchymal Cells in Interfacial Polyelectrolyte Complex Fibers Biomaterials · 2013
    10. Scalable Production of Controllable Dermal Papilla Spheroids on PVA Surfaces and the Effects of Spheroid Size on Hair Follicle Regeneration Biomaterials · 2012
    11. Fully Functional Hair Follicle Regeneration Through the Rearrangement of Stem Cells and Their Niches Nature Communications · 2012
    12. Clonal Growth of Dermal Papilla Cells in Hydrogels Reveals Intrinsic Differences Between Sox2-Positive and Sox2-Negative Cells In Vitro and In Vivo Journal of Investigative Dermatology · 2011
    13. Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay Journal of Investigative Dermatology · 2011
    14. De Novo Formation and Ultra-Structural Characterization of a Fiber-Producing Human Hair Follicle Equivalent In Vitro Journal of biotechnology · 2011
    15. Large-Scale Cultivation of Transplantable Dermal Papilla Cellular Aggregates Using Microfabricated PDMS Arrays Acta Biomaterialia · 2010
    16. Review of Hair Follicle Dermal Cells Journal of Dermatological Science · 2009
    17. Hair Follicle Neogenesis Induced by Cultured Human Scalp Dermal Papilla Cells Regenerative Medicine · 2009
    18. A Human Folliculoid Microsphere Assay for Exploring Epithelial-Mesenchymal Interactions in the Human Hair Follicle ˜The œjournal of investigative dermatology/Journal of investigative dermatology · 2008
    19. Scratching the Surface of Skin Development Nature · 2007
    20. Bioengineering the Hair Follicle Organogenesis · 2007
    21. Organogenesis From Dissociated Cells: Generation of Mature Cycling Hair Follicles From Skin-Derived Cells Journal of Investigative Dermatology · 2005
    22. Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla Journal of Investigative Dermatology · 2003
    23. Immunofluorescence Analysis of the Basement Membrane Zone Components in Human Anagen Hair Follicles Experimental Dermatology · 2003
    24. Plasticity and Cytokinetic Dynamics of the Hair Follicle Mesenchyme: Implications for Hair Growth Control Journal of Investigative Dermatology · 2003
    25. Simple and Rapid Method to Isolate and Culture Follicular Papillae from Human Scalp Hair Follicles Experimental Dermatology · 2002
    26. Molecular Mechanisms Regulating Hair Follicle Development ˜The œjournal of investigative dermatology/Journal of investigative dermatology · 2002
    27. Differences in Hair Follicle Dermal Papilla Volume Are Due to Extracellular Matrix Volume and Cell Number: Implications for the Control of Hair Follicle Size and Androgen Responses Journal of Investigative Dermatology · 1999
    28. The Psychosocial Consequences of Androgenetic Alopecia: A Review of the Research Literature British Journal of Dermatology · 1999
    29. The Biology of Hair Follicles The New England Journal of Medicine · 1999

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    2. Multi-View Light Sheet Fluorescence Microscopy for Imaging Cellular Self-Assembly in Spheroids of Human Hair Follicle Dermal Papilla Cells and Keratinocytes Journal of Investigative Dermatology · 2020
    3. Hedgehog Stimulates Hair Follicle Neogenesis by Creating Inductive Dermis During Murine Skin Wound Healing Nature Communications · 2018
    4. Hair-Follicle Dermal Papilla and Sheath Fibroblasts Provide a Supportive Microenvironment for Human Skin Regeneration British journal of dermatology/British journal of dermatology, Supplement · 2017
    5. Hair Follicle Regeneration In Vitro Paleontological Journal · 2016
    6. Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroids of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration ACS Applied Materials & Interfaces · 2016
    7. Beta-Catenin Activity in the Dermal Papilla Regulates Morphogenesis and Regeneration of Hair Developmental Cell · 2010
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