Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroids of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration

    February 2016 in “ ACS Applied Materials & Interfaces
    Bojie Lin, Yong Miao, Jin Wang, Zhiqi Hu, Lijuan Du, Su Yongsheng, Bingcheng Liu, Malcolm Xing
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    TLDR Technique creates 3D cell spheroids for hair-follicle regeneration.
    This document describes a technique for producing 3D spheroids of high-passaged human dermal papilla cells using surface tension guided hanging-drop. The spheroids can be used to form inductive microtissues for hair-follicle regeneration. The study found that the spheroids exhibited greater expression of signature genes and proteins associated with hair-inductivity compared to 2D cultured cells. The DP microtissues induced hair follicle neogenesis in vivo when co-transplanted with epidermal cells in nude mice. The study suggests that DP microtissues could be a promising approach for hair-follicle regeneration.
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