Controllable Production of Transplantable Adult Human High-Passage Dermal Papilla Spheroids Using 3D Matrigel Culture

This review discusses recent advances in hair follicle biology, regeneration, and tissue engineering, highlighting emerging therapeutic opportunities, and reports no new experimental results.

This study found that both human and rat dermal papilla spheroids can induce hair follicle neogenesis, but larger spheroids increase inductivity without significantly affecting hair fiber diameter.

This review summarizes current and emerging treatments for common hair loss conditions, highlighting good evidence for androgenetic alopecia but noting the lack of long-term data for alopecia areata and cicatricial alopecias.

This study reported that a three-dimensional hydrogel culture system supports the growth and maintenance of distinct dermal papilla cell types, crucial for skin reconstitution assays in neonatal mice.

This study found that cultured human dermal papilla cells formed into three-dimensional spheres have an increased ability to induce hair follicles from mouse epidermal cells compared to two-dimensional cultures.

This study achieved a first by successfully forming large quantities of dermal papilla spheroidal microtissues, which retained their hair induction potential, using a micropatterned culture system.

This review discusses the role of dermal cells in hair follicle regeneration and morphogenesis and highlights their potential therapeutic implications, but it reports no new empirical findings.

This study suggests that skin-derived precursors from Sox2(+) hair follicle dermal cells have the potential to function as dermal stem cells, supporting hair morphogenesis, dermal maintenance, and wound-healing.

This review discusses the development and advances in studying trichogenic dermal cells for hair follicle morphogenesis, summarizing methods, bioassays, and molecular markers, but reports no new research findings.

In this study, fibronectin coating on a low-adhesive substratum increased cell aggregation, growth, and motility, enhancing the formation of hair follicle dermal papilla spheroids.

This study presents a novel in vitro assay using human folliculoid microspheres to research hair growth, which may facilitate preclinical testing of hair growth-modulatory agents.

This study found that poly(ethylene-co-vinyl alcohol) membranes support the self-assembly of dermal papilla cells into compact spheroidal microtissues capable of inducing new hair follicles, suggesting potential for large-scale production for hair follicle regeneration.

This study reports that versican expression plays a crucial role in maintaining the anagen phase and hair inductive properties, as shown in a transgenic mouse model and hair growth assays.

This study developed molecular signatures for dermal papilla cells and their niche, uncovering novel signaling regulators and genes linked to hair disorders, which may inform future hair development research.

This study describes a simplified method for isolating and cultivating human hair follicle papillae that can be easily executed by new lab personnel.

This review discusses the psychological and social impacts of androgenetic alopecia and suggests that medical treatments can have some positive psychological effects, highlighting the condition's moderate stress and impact on body image.

This article discusses the biologic and psychosocial significance of hair, the current limitations in hair growth drugs, and anticipates future therapies based on advancing hair follicle research.

This study observed that cultured dermal papilla cells showed distinct functional properties from dermal fibroblasts, including lower proliferation rates, higher adhesion to collagen type IV, and different cell-matrix interactions.