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This study found that a 3D Matrigel culture technique for dermal papilla cell spheroids can enhance hair follicle inductivity and induce hair-like fiber differentiation in vitro, even with high-passage cells.

This study found that using three-dimensional cell culture with Matrigel enhances the biological function and stemness of stem cells, leading to improved soft tissue repair and healing by activating the host microenvironment and autologous stem cells.

This study successfully developed 3D hair follicle organoids from neonatal mouse epidermal and dermal cells, showing structural and molecular resemblance to native anagen hair follicles.

This review discusses recent advancements and ongoing challenges in engineering human skin with appendages using scaffold-free and scaffold-based bioengineering approaches, but it reports no new clinical results.

This study developed a biomimetic 3-D co-culture system that enhances the hair-inductive properties of human dermal papilla cell aggregates, potentially improving hair follicle tissue engineering.