Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

August 2011 in “ Journal of Investigative Dermatology ”

Byungjun Kang, Mi Hee Kwack, Moon Kyu Kim, Jung Chul Kim, Young Kwan Sung

sphere formation dermal papilla cells hair follicles reconstitution assay 2D culture epidermal cells

Studysummary This study found that cultured human dermal papilla cells formed into three-dimensional spheres have an increased ability to induce hair follicles from mouse epidermal cells compared to two-dimensional cultures.

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In 2011, a study found that sphere formation significantly enhanced the hair-inducing capacity of cultured human dermal papilla (DP) cells. The researchers used a reconstitution assay and expanded the DP cells in a two-dimensional (2D) culture. They observed hair follicle formation when DP spheres from various passages of culture were mixed with newborn mouse epidermal cells, but not when 2D cultures from the same population were used.

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Research cited in this study

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research Organogenesis From Dissociated Cells: Generation of Mature Cycling Hair Follicles From Skin-Derived Cells

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