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    Did you mean 2D cell culture?
    Glossary 2D cell culture

    cells grown on flat surfaces for research purposes

    2D cell culture, also known as two-dimensional cell culture, is a laboratory technique where cells are grown on a flat, typically plastic, surface such as a petri dish. This method allows researchers to study cell behavior, drug responses, and biological processes in a controlled environment, but it may not fully replicate the complex interactions found in a living organism.

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    1. 600 Differential Molecular Signature of Dermal Papilla Cells (DPCs) from Balding and Non balding Scalp in 2D, 3D culture and Co-culture with Adipose-derived stem cells (ASCs) Journal of Investigative Dermatology · 2019
    2. Comparative analysis of 3D and 2D cell-culturing methods in hair follicle spheroid morphogenesis and drug responsiveness Biomaterials Advances · 2025 · 1 citations
    3. Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth Frontiers in Cell and Developmental Biology · 2021 · 5 citations
    4. Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth Proceedings of the National Academy of Sciences of the United States of America · 2013 · 256 citations
    5. Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay Journal of Investigative Dermatology · 2011 · 75 citations
    6. Novel in vitro culture condition improves the stemness of human dermal stem/progenitor cells Molecules and Cells · 2013 · 18 citations
    7. RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture Journal of Cellular and Molecular Medicine · 2020 · 6 citations
    8. Culture Strategy Determines the Differentiation Status of Sweat Gland Cells Cells · 2025
    9. Peer Review #1 of "Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy (v0.1)" 2018
    10. Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-caffeoylquinic acid (TCQA) Research Square (Research Square) · 2021
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