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Glossary2D cell culture

cells grown on flat surfaces for research purposes

2D cell culture, also known as two-dimensional cell culture, is a laboratory technique where cells are grown on a flat, typically plastic, surface such as a petri dish. This method allows researchers to study cell behavior, drug responses, and biological processes in a controlled environment, but it may not fully replicate the complex interactions found in a living organism.

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Differential Molecular Signature of Dermal Papilla Cells from Balding and Non-Balding Scalp in 2D, 3D Culture and Co-Culture with Adipose-Derived Stem Cells

research Differential Molecular Signature of Dermal Papilla Cells from Balding and Non-Balding Scalp in 2D, 3D Culture and Co-Culture with Adipose-Derived Stem Cells

September 2019 in “Journal of Investigative Dermatology”

Dermal Papilla Cells grown in 3D and with stem cells better mimic natural hair growth conditions than cells grown in 2D.

Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth

research Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth

5 citations , September 2021 in “Frontiers in Cell and Developmental Biology”

Dihydrotestosterone treatment on 2D and 3D-cultured skin cells slows down hair growth by affecting certain genes and could be a potential target for hair loss treatment.

research RNA Sequence Analysis of Dermal Papilla Cells’ Regeneration in 3D Culture

6 citations , October 2020 in “Journal of Cellular and Molecular Medicine”

3D culture helps maintain hair growth cells better than 2D culture and identifies key genes for potential hair loss treatments.

Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid

research Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid

November 2021 in “Research Square (Research Square)”

3D spheroid cultures of human hair follicle cells are better for hair growth research than 2D cultures, and they provide new insights into how hair growth treatments like minoxidil and TCQA work.

Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

research Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

75 citations , August 2011 in “Journal of Investigative Dermatology”

Forming spheres boosts the ability of certain human cells to create hair follicles when mixed with mouse skin cells.

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community 137 years old and still far more hair than the average r/tressless user

in Satire 1069 upvotes 2 years ago

The post and conversation are about a user claiming to be 137 years old with a full head of hair. Specific treatments mentioned include dutasteride, RU58841, and minoxidil.

community 11mth daily 0.5mg oral finasteride

in Finasteride/Dutasteride 826 upvotes 11 months ago

The user experienced significant hair regrowth with 0.5mg oral finasteride daily, despite a minoxidil allergy, and reported no side effects. They also used a keto shampoo and were satisfied with the results.

community Are topicals with caffeine useful to promote hairgrowth?

in Research/Science 3 upvotes 1 year ago

The user is asking if caffeine topicals are effective for hair growth, noting they already use Minoxidil and finasteride. They are unsure if caffeine topicals are beneficial or a waste of money.

community Finasteride and Eye Problems: You’re Not Going Crazy

in Finasteride/Dutasteride 503 upvotes 5 months ago

Finasteride can cause dry eyes and vision issues due to its impact on the meibomian glands. Users have mixed experiences, with some experiencing eye discomfort and others having no side effects.

community PP405 Drug in Action: Picture From Official Company Website

in Technology 781 upvotes 10 months ago

PP405 shows promise for hair follicle reactivation with minimal side effects. Users recommend continuing Minoxidil and Finasteride until more results are confirmed.

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