RNA Sequence Analysis of Dermal Papilla Cells’ Regeneration in 3D Culture
October 2020
in “
Journal of Cellular and Molecular Medicine
”
dermal papilla cells DPCs hair follicle growth differentially expressed genes DEGs IL1B CXCL12 HGF EGFR APP CCL2 PTGS2 MMP9 NGF SPP1 Gene Ontology GO Kyoto Encyclopedia of Genes and Genomes KEGG protein-protein interaction PPI 3D culture 2D culture regenerative properties hair growth mechanisms hair regeneration therapies
TLDR 3D culture helps maintain hair growth cells better than 2D culture and identifies key genes for potential hair loss treatments.
The study focused on the molecular mechanisms of dermal papilla cells (DPCs) regeneration in 3D culture, which is essential for hair follicle growth. Researchers compared DPCs in 2D culture at passage 2 and passage 8 with DPCs in 3D culture at passage 8. They identified 2001 differentially expressed genes (DEGs), including 1642 known and 359 novel genes, and highlighted 10 hub genes (IL1B, CXCL12, HGF, EGFR, APP, CCL2, PTGS2, MMP9, NGF, and SPP1) using analyses such as Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI). The study found that 3D culture could maintain the regenerative properties of DPCs, unlike 2D culture, and suggested that the identified hub genes and pathways could provide insights into hair growth mechanisms and potential targets for hair regeneration therapies. Further research was recommended to confirm these findings.
