Wnt1a Maintains Characteristics of Dermal Papilla Cells That Induce Mouse Hair Regeneration in a 3D Preculture System

June 2015 in “ Journal of Tissue Engineering and Regenerative Medicine ”

Liang Dong, Haojie Hao, Jiejie Liu, Chuan Tong, Dongdong Ti, Deyun Chen, Li Chen, Meirong Li, Huiling Liu, Xiaobing Fu, Weidong Han

Wnt1a dermal papilla cells 3D culture system conditioned medium bone marrow mesenchymal stem cells Wnt-CM epidermal cells collagen-chitosan scaffold dermal condensates proliferative cell layer hair regeneration nude mice culture expansion DP cells EP cells CCS

TLDR Wnt1a helps keep cells that can grow hair effective for potential hair loss treatments.

The study from June 29, 2015, explored the use of Wnt1a to preserve the hair-inducing properties of dermal papilla (DP) cells in a 3D culture system. Researchers used a conditioned medium from Wnt1a-expressing bone marrow mesenchymal stem cells (Wnt-CM) to treat DP cells, which were then combined with epidermal (EP) cells in a collagen-chitosan scaffold (CCS). This combination formed dermal condensates and a proliferative cell layer in vitro. When transplanted into nude mice, the CCS cells induced hair regeneration. The study concluded that Wnt-CM effectively maintains the hair induction ability of DP cells during culture expansion, suggesting its potential for large-scale preparation of cells for hair loss treatment. However, the number of mice used in the study was not mentioned.

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