Regeneration of a Bioengineered 3D Integumentary Organ System from iPS Cells

April 2019 in “ Nature protocols ”

Koh-éi Toyoshima, Miho Ogawa, Takashi Tsuji

3D integumentary organ system iPS cells embryoid bodies Wnt10b clustering-dependent embryoid body transplantation hair follicles sebaceous glands pluripotent stem cells 3D-IOS induced pluripotent stem cells EBs CDB transplantation

TLDR Scientists created a functional 3D skin system from stem cells that can be transplanted into wounds.

The document described a method to generate a 3D integumentary organ system (3D-IOS) from murine induced pluripotent stem (iPS) cells. The process involved growing iPS cells for 7 days to form embryoid bodies (EBs), stimulating these EBs with Wnt10b, and then transplanting them in vivo using a clustering-dependent embryoid body (CDB) transplantation method. After 30 days, the EBs differentiated into a 3D-IOS containing mature hair follicles and sebaceous glands, which could be transplanted into wounds in other mice. The transplanted 3D-IOS demonstrated full physiological function 14 days post-transplantation, showcasing the potential for creating functional organ systems from pluripotent stem cells.

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Research cited in this study

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research Bioengineering a 3D Integumentary Organ System from iPS Cells Using an In Vivo Transplantation Model

77 citations, April 2016 in “Science Advances”

Researchers created a fully functional, bioengineered skin system with hair from stem cells that successfully integrated when transplanted into mice.

research Advances in Skin Grafting and Treatment of Cutaneous Wounds

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research Fully Functional Hair Follicle Regeneration Through the Rearrangement of Stem Cells and Their Niches

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research The Hair Follicle as a Dynamic Mini-Organ

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research Integument Pattern Formation Involves Genetic and Epigenetic Controls: Feather Arrays Simulated by Digital Hormone Models

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Feather patterns form through genetic and epigenetic controls, with cells self-organizing into periodic patterns.

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research Regeneration of a Bioengineered 3D Integumentary Organ System from iPS Cells

12 citations, April 2019 in “Nature protocols”

Scientists created a functional 3D skin system from stem cells that can be transplanted into wounds.

Regeneration of a Bioengineered 3D Integumentary Organ System from iPS Cells

Research cited in this study

research Bioengineering a 3D Integumentary Organ System from iPS Cells Using an In Vivo Transplantation Model

research Advances in Skin Grafting and Treatment of Cutaneous Wounds

research Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ

research Fully Functional Hair Follicle Regeneration Through the Rearrangement of Stem Cells and Their Niches

research The Hair Follicle as a Dynamic Mini-Organ

research Scratching the Surface of Skin Development

research Integument Pattern Formation Involves Genetic and Epigenetic Controls: Feather Arrays Simulated by Digital Hormone Models

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