Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid
November 2021
in “
Research Square (Research Square)
”
TLDR 3D spheroid cultures of human hair follicle cells are better for hair growth research than 2D cultures, and they provide new insights into how hair growth treatments like minoxidil and TCQA work.
The study demonstrates that 3D spheroid cultures of human hair follicle dermal papilla cells (HFDPC) provide a more effective model for hair growth research than traditional 2D cultures. The 3D model was found to influence not only hair growth-related pathways but also immune system-related pathways. The study also compared the expression of signaling molecules and metabolism-associated proteins in HFDPC treated with minoxidil, an FDA-approved drug for hair loss treatment, and 3,4,5-tri-O-caffeoylquinic acid (TCQA), a compound recently found to induce hair growth, in both 3D and 2D cell cultures. The results validate the suitability of this cell line for 3D modeling and provide new insights into the mechanisms behind the hair growth effects of 3D spheroids treated with hair growth promoting agents.
