Neonatal Murine Skin-Derived Cells Transplanted Using a Mini-Chamber Model Produce Robust and Normal Hair

In the 2013 study, researchers successfully used a mini-chamber model to transplant neonatal murine skin-derived cells, resulting in the production of robust and normal hair in nude mice. This model was more efficient than traditional methods, requiring fewer cells and allowing multiple transplants per animal, with a high graft take rate. Freshly isolated neonatal murine dermal cells and those cultured overnight were both effective in reconstructing hair follicles, while human scalp follicle cells failed to do so. The density of hair reconstitution was dependent on the number of cells transplanted. The reconstituted hair follicles were confirmed to be formed by the grafted cells and were capable of long-term survival and regeneration after plucking. The study concluded that the mini-chamber model is a reliable and cost-effective method for hair follicle reconstitution studies and could be useful for future hair regeneration therapies.