Hair Follicle Regeneration Using Grafted Rodent and Human Cells

April 2007 in “ Journal of Investigative Dermatology ”

Ritsuko Ehama, Yumiko Ishimatsu-Tsuji, Shunsuke Iriyama, Ritsuro Ideta, Tsutomu Soma, Kiichiro Yano, Chikako Kawasaki, Satoshi O. Suzuki, Yuji Shirakata, Koji Hashimoto, Jiro Kishimoto

hair follicle regeneration grafted rodent cells grafted human cells human keratinocytes murine mesenchymal cells epithelial-mesenchymal interaction glabrous skin follicular epithelia adult epidermis chimeric hair follicle-like structures undifferentiated stem-like cells follicular epithelium culture conditions inductive property grafted cells keratinocytes mesenchymal cells stem cells skin cells

Studysummary In this study, co-grafting human keratinocytes with murine dermal papilla-enriched cells produced hair follicle-like structures, but without regular hair formation, suggesting anomalous folliculogenesis.

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The study aimed to regenerate hair follicles using grafted rodent and human cells. The researchers found that hair follicle-like structures consisting of human keratinocytes and murine mesenchymal cells were generated, indicating that EMI function to a certain extent even between human and mouse cells. They also demonstrated that keratinocytes from glabrous skin can differentiate into follicular epithelia. However, the efficiency of adult epidermis generating chimeric hair follicle-like structures was lower than newborn foreskins, implying that there are only a small number of undifferentiated stem-like cells that have the potential to differentiate into follicular epithelium, and that number decreases with maturation. The study suggests that identifying suitable culture conditions and/or factors required to maintain the inductive property are high priorities for investigation.

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