In Vitro Induction of Hair Follicle Signatures Using Human Dermal Papilla Cells Encapsulated in Fibrin Microgels

August 2023 in “ Cell Proliferation ”

Cristina Quílez, Leticia Valencia, Jorge Gonzalez-Rico, Leticia Suárez-Cabrera, Lidia Amigo‐Morán, José L. Jorcano, Diego Velasco

human follicle dermal papilla cells fibrin microgels dermal papilla spheroids cell viability epidermal invaginations hair follicle regeneration therapies hFDPc FM DPS

Studysummary This study observed that incorporating human follicle dermal papilla cells into fibrin microgels increased cell viability and the formation of hair follicle structures in in vitro skin cultures compared to traditional dermal papilla spheroids, suggesting a promising approach for hair follicle regeneration therapies. Our plain-language summary of this paper — not a Tressless recommendation.

The study explored the use of human follicle dermal papilla cells (hFDPc) encapsulated in plasma-derived fibrin microgels (FM) as an alternative to dermal papilla spheroids (DPS) for culture systems. The research found that FM had similar morphology to DPS and DP structure, and cell viability was higher in FM than DPS, with values between 85% and 40% for the lowest and highest cell concentrations. FM also restored hFDPc intrinsic properties and induced epidermal invaginations. The DPS method showed a mean efficiency of 17.4%, while FM had a mean efficiency of 48.9%. The study concluded that FM could be a potential candidate for hFDPc encapsulation to mimic the human DP microenvironment, and is a significant step towards the development of human hair follicle regeneration therapies.

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Research cited in this study

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research As a Carrier–Transporter for Hair Follicle Reconstitution, Platelet-Rich Plasma Promotes Proliferation and Induction of Mouse Dermal Papilla Cells

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