In Vitro Induction of Hair Follicle Signatures Using Human Dermal Papilla Cells Encapsulated in Fibrin Microgels

August 2023 in “ Cell Proliferation ”

Cristina Quílez, Leticia Valencia, Jorge Gonzalez-Rico, Leticia Suárez-Cabrera, Lidia Amigo‐Morán, José L. Jorcano, Diego Velasco

human follicle dermal papilla cells fibrin microgels dermal papilla spheroids cell viability epidermal invaginations hair follicle regeneration therapies hFDPc FM DPS

TLDR Human cells in plasma-derived gels can potentially mimic hair follicle environments, improving hair regeneration therapies.

The study explored the use of human follicle dermal papilla cells (hFDPc) encapsulated in plasma-derived fibrin microgels (FM) as an alternative to dermal papilla spheroids (DPS) for culture systems. The research found that FM had similar morphology to DPS and DP structure, and cell viability was higher in FM than DPS, with values between 85% and 40% for the lowest and highest cell concentrations. FM also restored hFDPc intrinsic properties and induced epidermal invaginations. The DPS method showed a mean efficiency of 17.4%, while FM had a mean efficiency of 48.9%. The study concluded that FM could be a potential candidate for hFDPc encapsulation to mimic the human DP microenvironment, and is a significant step towards the development of human hair follicle regeneration therapies.

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In Vitro Induction of Hair Follicle Signatures Using Human Dermal Papilla Cells Encapsulated in Fibrin Microgels

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research Injectable Platelet Rich Fibrin Facilitates Hair Follicle Regeneration by Promoting Human Dermal Papilla Cell Proliferation, Migration, and Trichogenic Inductivity

research Preparation of Hair Beads and Hair Follicle Germs for Regenerative Medicine

research Platelet-Rich Plasma for Androgenetic Alopecia: A Review of the Literature and Proposed Treatment Protocol

research Methods for the Isolation and 3D Culture of Dermal Papilla Cells from Human Hair Follicles

research As a Carrier–Transporter for Hair Follicle Reconstitution, Platelet-Rich Plasma Promotes Proliferation and Induction of Mouse Dermal Papilla Cells

research Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid

research Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

research Review of Hair Follicle Dermal Cells

research The Hair Follicle as a Dynamic Mini-Organ

research The Biology of Hair Follicles

research Distribution of Extracellular Matrix Molecules in Human Hair Follicles

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