Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold

April 2020 in “ Biomolecules ”

Nausika Betriu, Claire Jarrosson-Moral, Carlos E. Semino

3D self-assembling peptide scaffold RAD16-I human hair follicle dermal papilla cells HFDPC alkaline phosphatase versican corin osteogenic differentiation adipogenic differentiation tissue engineering hair loss treatments 3D scaffold peptide scaffold hair follicle cells hair-inducing markers bone differentiation fat differentiation hair loss

Studysummary The study suggests that a 3D culture system using the RAD16-I peptide scaffold can help restore the original phenotype of hair follicle dermal papilla cells and support their osteogenic and adipogenic differentiation. Our plain-language summary of this paper — not a Tressless recommendation.

The study explored the use of a 3D self-assembling peptide scaffold, RAD16-I, for culturing human hair follicle dermal papilla cells (HFDPC). The research aimed to address the loss of hair inductive potency that HFDPC experience in traditional 2D cultures. The results indicated that the 3D RAD16-I scaffold successfully restored key signature markers of HFDPC, such as alkaline phosphatase, versican, and corin, which are essential for their hair-inducing ability. Additionally, the scaffold supported the differentiation of HFDPC into osteogenic and adipogenic lineages when chemical inducers were present. This suggests that RAD16-I could be a promising platform for HFDPC culture, phenotype recovery, and differentiation, potentially benefiting tissue engineering and hair loss treatments.

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Research cited in this study

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research Characterization of Human Dermal Papilla Cells in Alginate Spheres

8 citations , October 2018 in “Applied sciences”

This study found that alginate spheres maintain dermal papilla cells in a dormant state with increased trichogenecity, suggesting potential benefits for cell therapy in androgenic alopecia.

research Multifaceted Role of Hair Follicle Dermal Cells in Bioengineered Skins

33 citations , September 2016 in “British journal of dermatology/British journal of dermatology, Supplement”

This study found that human hair follicle dermal cells, specifically dermal sheath cells, can serve as an alternative and potentially superior cell source for constructing the dermal component of bioengineered skin in both in vitro and in vivo settings.

Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroids of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration

research Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroids of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration

62 citations , February 2016 in “ACS Applied Materials & Interfaces”

This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.

research Controllable Production of Transplantable Adult Human High-Passage Dermal Papilla Spheroids Using 3D Matrigel Culture

46 citations , September 2014 in “Tissue engineering. Part A”

This study found that a 3D Matrigel culture technique for dermal papilla cell spheroids can enhance hair follicle inductivity and induce hair-like fiber differentiation in vitro, even with high-passage cells.

Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

research Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

256 citations , October 2013 in “Proceedings of the National Academy of Sciences of the United States of America”

This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.

Clonal Growth of Dermal Papilla Cells in Hydrogels Reveals Intrinsic Differences Between Sox2-Positive and Sox2-Negative Cells In Vitro and In Vivo

research Clonal Growth of Dermal Papilla Cells in Hydrogels Reveals Intrinsic Differences Between Sox2-Positive and Sox2-Negative Cells In Vitro and In Vivo

68 citations , December 2011 in “Journal of Investigative Dermatology”

This study reported that a three-dimensional hydrogel culture system supports the growth and maintenance of distinct dermal papilla cell types, crucial for skin reconstitution assays in neonatal mice.

Clonal Multipotency and Effect of Long-Term In Vitro Expansion on Differentiation Potential of Human Hair Follicle Derived Mesenchymal Stem Cells

research Clonal Multipotency and Effect of Long-Term In Vitro Expansion on Differentiation Potential of Human Hair Follicle Derived Mesenchymal Stem Cells

73 citations , August 2011 in “Stem Cell Research”

This study found that human hair follicle-derived mesenchymal stem cells can differentiate into myogenic, osteogenic, adipogenic, and chondrogenic lineages, although this potential decreases over time and varies by lineage.

research Review of Hair Follicle Dermal Cells

321 citations , December 2009 in “Journal of Dermatological Science”

This review discusses the role of dermal cells in hair follicle regeneration and morphogenesis and highlights their potential therapeutic implications, but it reports no new empirical findings.

research Hair Follicle Neogenesis Induced by Cultured Human Scalp Dermal Papilla Cells

63 citations , September 2009 in “Regenerative Medicine”

This study developed a method for expanding human dermal papilla cells in vitro while maintaining their ability to induce hair growth, advancing the potential for follicular cell implantation as a treatment for hair loss.

Self-Assembly of Dermal Papilla Cells into Inductive Spheroidal Microtissues on Poly(Ethylene-Co-Vinyl Alcohol) Membranes for Hair Follicle Regeneration

research Self-Assembly of Dermal Papilla Cells into Inductive Spheroidal Microtissues on Poly(Ethylene-Co-Vinyl Alcohol) Membranes for Hair Follicle Regeneration

80 citations , June 2008 in “Biomaterials”

This study found that poly(ethylene-co-vinyl alcohol) membranes support the self-assembly of dermal papilla cells into compact spheroidal microtissues capable of inducing new hair follicles, suggesting potential for large-scale production for hair follicle regeneration.

research Comparative Characterization of Hair Follicle Dermal Stem Cells and Bone Marrow Mesenchymal Stem Cells

151 citations , February 2006 in “Stem Cells and Development”

This study found that hair follicle-derived dermal stem cells and bone marrow mesenchymal stem cells have similar growth, differentiation capabilities, and surface marker expressions, suggesting hair follicles could be accessible sources for mesenchymal stem cells.

research Hair Cycle-Specific Expression of Versican in Human Hair Follicles

88 citations , May 2005 in “Journal of Dermatological Science”

In this study, specific versican expression was observed in the anagen phase of human hair follicles, suggesting its importance in maintaining normal hair growth.

research Follicular Cell Implantation: An Emerging Cell Therapy for Hair Loss

13 citations , May 2005 in “Seminars in Plastic Surgery”

This article discusses the cell therapy potential of follicular cell implantation for hair regeneration and reports no new clinical results, emphasizing the role of dermal papilla cells in forming new follicles.

research Cultured Cells From the Adult Human Hair Follicle Dermis Can Be Directed Toward Adipogenic and Osteogenic Differentiation

38 citations , April 2005 in “Journal of Investigative Dermatology”

Human hair follicle cells can become fat and bone cells, useful for therapy.

research Effect of Minoxidil on Proliferation and Apoptosis in Dermal Papilla Cells of Human Hair Follicle

149 citations , March 2004 in “Journal of Dermatological Science”

This study found that minoxidil stimulates hair follicle growth by promoting dermal papilla cell survival and activating ERK and Akt pathways, which leads to increased proliferation and reduced cell death.

research Hair Follicle Dermal Cells Differentiate Into Adipogenic and Osteogenic Lineages

268 citations , December 2003 in “Experimental Dermatology”

This study demonstrated that clonal dermal papilla and dermal sheath cell lines from hair follicles can differentiate into lipid and bone cells, suggesting their potential as a stem cell source for tissue engineering.

Activation of Cytoprotective Prostaglandin Synthase-1 by Minoxidil as a Possible Explanation for Its Hair Growth-Stimulating Effect

research Activation of Cytoprotective Prostaglandin Synthase-1 by Minoxidil as a Possible Explanation for Its Hair Growth-Stimulating Effect

81 citations , February 1997 in “Journal of Investigative Dermatology”

This study suggests that minoxidil activates PGHS-1, which may explain its hair growth-stimulating effects and could aid in designing more potent hair growth-promoting drugs.