Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion

January 2020 in “ Methods in molecular biology ”

Summik Limbu, Claire A. Higgins

dermal papilla cells hair follicles mesenchymal cells epithelial stem cells matrix cells melanocytes androgenetic alopecia hair shaft hair loss microdissection enzyme digestion DP cells hair roots stem cells hair thinning baldness

TLDR The method successfully isolates cells that are important for hair growth and could help study hair loss.

The document from 2020 detailed a method for isolating dermal papilla (DP) cells from human hair follicles. The DP, a cluster of mesenchymal cells at the base of the hair follicle, interacts with various other cell types within the follicle, such as epithelial stem cells, matrix cells, and melanocytes, and regulates their function. The size of the DP is directly proportional to the width of the hair shaft, and a decrease in both cell number and DP size is observed in hair loss conditions like androgenetic alopecia. However, microdissected ex vivo DP can instruct the growth of new hair follicles. The study of DP cells and their role in human hair growth has often been hampered by the technical challenge of DP isolation and culture, which this method aimed to address.

View this study on link.springer.com →

Discuss this study in the Community →

Research cited in this study

9 / 9 results

Demethylation of ITGAV Accelerates Osteogenic Differentiation in a Blast-Induced Heterotopic Ossification In Vitro Cell Culture Model

research Demethylation of ITGAV Accelerates Osteogenic Differentiation in a Blast-Induced Heterotopic Ossification In Vitro Cell Culture Model

11 citations , December 2018 in “Bone”

Removing a methyl group from the ITGAV gene speeds up bone formation in a specific type of bone disease model.

research Methods for the Isolation and 3D Culture of Dermal Papilla Cells from Human Hair Follicles

55 citations , April 2017 in “Experimental Dermatology”

The document describes a way to isolate and grow human hair follicle cells in 3D to help study hair growth.

research Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices

214 citations , April 2017 in “Cell”

Different small areas within hair follicles send specific signals that control what type of cells stem cells become.

research Hair Follicle: A Novel Source of Multipotent Stem Cells for Tissue Engineering and Regenerative Medicine

65 citations , November 2012 in “Tissue Engineering Part B-reviews”

Hair follicle stem cells are a promising source for tissue repair and treating skin or hair diseases.

research Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle

417 citations , September 2005 in “PLoS biology”

Understanding gene expression in hair follicles can reveal insights into hair growth and disorders.

research Simple and Rapid Method to Isolate and Culture Follicular Papillae from Human Scalp Hair Follicles

78 citations , August 2002 in “Experimental Dermatology”

Researchers developed a quick and easy way to get and grow cells from the base of human hair follicles.

research Differences in Hair Follicle Dermal Papilla Volume Are Due to Extracellular Matrix Volume and Cell Number: Implications for the Control of Hair Follicle Size and Androgen Responses

231 citations , December 1999 in “Journal of Investigative Dermatology”

Hair follicle size is mainly influenced by the number of cells and extracellular matrix volume, with cell number having a larger impact.

research Improved Method for the Isolation and Cultivation of Human Scalp Dermal Papilla Cells

45 citations , May 1992 in “Journal of Investigative Dermatology”

research Distribution of Extracellular Matrix Molecules in Human Hair Follicles

45 citations , December 1991 in “Annals of the New York Academy of Sciences”

ECM molecules are crucial for hair growth and development.

Related Community Posts Join

6 / 1000+ results

Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

community Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

in Research 865 upvotes 1 year ago

The treatment for androgenetic alopecia involves using finasteride and minoxidil with intense exercise and cold exposure to boost metabolism and reduce androgenic effects, potentially leading to hair regrowth. This approach may activate biological pathways for improved hair and overall health.

community If You Have DUPA, PLEASE READ THIS: Everyone Should Be Scalp Biopsied

in Research 830 upvotes 1 year ago

Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.

community The Real Cause Of Androgenetic Alopecia

in Research/Science 682 upvotes 1 year ago

Androgenetic alopecia is caused by DHT affecting hair growth. Finasteride and minoxidil are used to manage hair loss by blocking DHT and promoting hair growth.

community New and Interesting HairLoss Studies/Papers/Reviews

in Treatment 44 upvotes 5 years ago

Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

community Comprehensive Microneedling Guide

in Microneedling 290 upvotes 3 years ago

Microneedling for hair restoration involves daily use of a 0.3 mm device and weekly use of a 0.5 mm device. Combining microneedling with Minoxidil can enhance hair growth.

community HairClone: Good info from their site

in Technology 10 upvotes 3 years ago

HairClone aims to rejuvenate miniaturizing hair follicles through follicle banking and cell expansion, with treatments potentially available in the UK by 2022. The process involves extracting, storing, and cloning hair follicles, but full regenerative treatments will take many years to develop.

Related Research

7 / 7 results

research Possible Cellular Communication with Human Follicle Dermal Papilla Cells via Secretions from Human Hair Follicle Keratinocytes

January 2022 in “Journal of St. Marianna University”

Substances from human hair cells can affect hair loss-related genes, potentially leading to new treatments for baldness.

research A Model System to Analyze the Ability of Human Keratinocytes to Form Hair Follicles

15 citations , April 2014 in “Experimental Dermatology”

Scientists developed a system to study human hair growth using skin cells, which could help understand hair development and improve skin substitutes for medical use.

research Review of Hair Follicle Dermal Cells

321 citations , December 2009 in “Journal of Dermatological Science”

Dermal cells are key in controlling hair growth and could potentially be used in hair loss treatments, but more research is needed to improve hair regeneration methods.