TLDR Mouse hair follicle stem cells were successfully isolated and used to regenerate hair follicles with two different methods.
The document from 2016 detailed a method for isolating hair follicle bulge stem cells (HFSCs) from mice and evaluating their ability to regenerate hair follicles using two different assays: the patch assay and the silicone chamber assay. The HFSCs were shown to be capable of regenerating de novo hair follicles and repopulating all cell lineages within these follicles. The patch assay was described as easier and more cost-effective, but with limitations such as uncontrolled hair shaft orientation and improper hair cycling. In contrast, the chamber assay, though more labor-intensive, resulted in correctly oriented hairs with normal cycling. The study provided protocols for isolating HFSCs, performing cell sorting, and preparing cells for reconstitution assays, highlighting the potential of HFSCs in regenerative medicine. However, the document did not specify the number of mice used or the success rates of the assays.
24 citations
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August 2011 in “Experimental Dermatology” The flap assay grows the most natural hair but takes the longest, the chamber assay is hard work but gives dense, normal hair, and the patch assay is quick but creates poorly oriented hair with some issues.
427 citations
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April 2008 in “Nature Protocols” 
205 citations
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April 2005 in “Journal of Investigative Dermatology” Scientists have found a way to create hair follicles from skin cells of newborn mice, which can grow and cycle naturally when injected into adult mouse skin.
387 citations
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November 2003 in “Journal of Investigative Dermatology” The K15 promoter effectively targets stem cells in the hair follicle bulge.
561 citations
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April 2003 in “Journal of Investigative Dermatology”
56 citations
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May 2017 in “Nature Cell Biology” Hair can regrow after certain stem cells are lost because other stem cells can take over their role.
7 citations
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January 2016 in “Methods in molecular biology” Mouse hair follicle stem cells were successfully isolated and used to regenerate hair follicles with two different methods.
36 citations
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October 2015 in “Cell reports” Gab1 protein is crucial for hair growth and stem cell renewal, and Mapk signaling helps maintain these processes.
65 citations
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November 2012 in “Tissue Engineering Part B-reviews” Hair follicle stem cells are a promising source for tissue repair and treating skin or hair diseases.
260 citations
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June 2011 in “Cell” Wnt signaling is crucial for pigmented hair regeneration by controlling stem cell activation and differentiation.
35 citations
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April 2008 in “Human Molecular Genetics” Skin and hair can help us understand organ regeneration, especially how certain stem cells might be used to form new organs.
