Microscopy-Guided Laser Ablation for the Creation of Complex Skin Models with Folliculoid Appendages

October 2020 in “ Bioengineering & translational medicine ”

Carla M. Abreu, Luca Gasperini, Manuela E.L. Lago, Rui L. Reis, A. P. Marques

Studysummary This study demonstrates the use of microscopy-guided laser ablation to create a highly structured 3D hydrogel-based organotypic skin model with folliculoid structures, potentially useful for studying hair follicle development or bioactive substance screening.

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The study demonstrated that microscopy-guided laser ablation (MGLA) could create complex skin models with folliculoid appendages by forming 3D microchannels in fibroblast-laden hydrogels. These channels facilitated the interaction of keratinocytes (KCs) and dermal papilla (DP) cells, leading to the formation of follicular-like structures. Despite some cell death near the ablation sites, overall cell viability was maintained, and the resulting structures exhibited complex epithelial organization and marker expression similar to human hair follicles. This advanced biofabrication technique provided a sophisticated in vitro model for studying hair follicle development and could be useful for cosmetic testing and drug development.

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research Microscopy-Guided Laser Ablation for the Creation of Complex Skin Models with Folliculoid Appendages

5 citations , October 2020 in “Bioengineering & translational medicine”

This study demonstrates the use of microscopy-guided laser ablation to create a highly structured 3D hydrogel-based organotypic skin model with folliculoid structures, potentially useful for studying hair follicle development or bioactive substance screening.

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33 citations , September 2016 in “British journal of dermatology/British journal of dermatology, Supplement”

This study found that human hair follicle dermal cells, specifically dermal sheath cells, can serve as an alternative and potentially superior cell source for constructing the dermal component of bioengineered skin in both in vitro and in vivo settings.

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256 citations , October 2013 in “Proceedings of the National Academy of Sciences of the United States of America”

This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.

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85 citations , September 2013 in “International Journal of Molecular Sciences”

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research Changes in Fibronectin, Laminin, and Type IV Collagen Distribution Relate to Basement Membrane Restructuring During the Rat Vibrissa Follicle Hair Growth Cycle

45 citations , August 1992 in “PubMed”

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research Epithelial Growth by Rat Vibrissae Follicles In Vitro Requires Mesenchymal Contact via Native Extracellular Matrix

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