Microscopy-Guided Laser Ablation for the Creation of Complex Skin Models with Folliculoid Appendages
December 2020
in “
Bioengineering & translational medicine
”
microscopy-guided laser ablation MGLA folliculoid appendages 3D microchannels fibroblast-laden hydrogels keratinocytes dermal papilla cells follicular-like structures epithelial organization hair follicles biofabrication in vitro model hair follicle development laser ablation 3D channels fibroblast hydrogels KCs DP cells hair-like structures skin models hair growth study
TLDR Researchers used a laser to create advanced skin models with hair-like structures.
The study demonstrated that microscopy-guided laser ablation (MGLA) could create complex skin models with folliculoid appendages by forming 3D microchannels in fibroblast-laden hydrogels. These channels facilitated the interaction of keratinocytes (KCs) and dermal papilla (DP) cells, leading to the formation of follicular-like structures. Despite some cell death near the ablation sites, overall cell viability was maintained, and the resulting structures exhibited complex epithelial organization and marker expression similar to human hair follicles. This advanced biofabrication technique provided a sophisticated in vitro model for studying hair follicle development and could be useful for cosmetic testing and drug development.
