Biofabrication of Human Skin with Its Appendages

This study found that a hydrogel containing icariin enhanced wound healing and hair-follicle regeneration in vivo by promoting M2 macrophage polarization and modulating the BMP pathway, with treated skin defects showing improved recovery and new hair follicle development compared to untreated defects.

This review discusses human hair follicle development and highlights the potential of creating human in vitro models and skin substitutes with hair follicles, reporting no new clinical results.

This review discusses potential cell sources and bioengineering strategies for regenerating hair follicles with functional cycling, reporting no new results.

Dermal exosomes with miR-218-5p boost hair growth by controlling β-catenin signaling.

Scientists created human skin with hair from stem cells, which could help treat hair loss and skin conditions.

This study demonstrates that incorporating human neopapilla into reconstructed human skin can initiate hair follicle morphogenesis by stimulating epidermal invagination and maintaining follicle-inductive properties in vitro.

This article reviews current strategies and advancements in regenerating skin appendages and sensory nerves in tissue-engineered skin, highlighting the challenge of restoring skin sensations like pain, temperature, and touch to improve patients’ quality of life.

This study reported that a novel chitosan/polyvinyl alcohol nanofiber sponge effectively enhances the hair follicle-inducing ability of dermal papilla multicellular spheroids in a mouse model.

This study found that their system effectively supports the rapid formation of human hair follicle organoids, providing a platform for studying hair regeneration and drug screening.

This study found that the human placenta extracellular matrix hydrogel restored the hair-inductive capacity of high-passaged dermal papilla cells, enabling them to regenerate new hair follicles when co-grafted with mouse epidermal cells.

This review examines the role of epidermal stem cells in wound healing, discussing their characteristics and mechanisms, but does not report new clinical results.

In this study, researchers developed a chemical cocktail and suspension culture method to transform human dermal fibroblasts into dermal papilla-like cells capable of inducing hair follicle formation in vitro and in vivo.

This study found that Blimp1+ cells from mice can generate sebaceous gland organoids in vitro, which mimic natural gland traits and may aid in studying acne vulgaris.

This review discusses various hormones and signaling pathways involved in sebaceous gland homeostasis and their potential impact on acne pathogenesis, but it reports no new experimental findings.

This study developed an in vitro system to analyze human skin progenitor cells' ability to form hair peg-like structures, providing insights for engineering hair follicle organoids.

This study demonstrated that enhancing human skin constructs with hair follicles through engineered cell organization and vascularization improved hair growth in immunodeficient mice, suggesting potential advances for treating alopecia and chronic wounds.

In this study, researchers successfully derived skin organoids from mouse pluripotent stem cells that spontaneously produce hair follicles, potentially aiding in hair growth research and in vitro drug testing.

This review discusses the limitations of animal models, particularly mice, in accurately predicting human skin wound healing outcomes and emphasizes understanding species-specific differences in skin characteristics for better translation to clinical settings.

This study reports that using nanogel and layer-by-layer self-assembly to encapsulate single dermal papilla cells can form cell spheroids that regenerate hair follicles successfully in a hair follicle regeneration model.

This study found that newborn mouse skin organoids can robustly grow hair in vivo, while adult organoids require specific interventions to restore their hair-forming ability.

This study established a new in vitro culture system that supports the growth and dynamic self-organization of hair follicle stem cells and their progeny, promoting a stable population equilibrium.

This study found that hair follicles can be used to generate pluripotent stem cells, which successfully differentiate into keratinocytes and may improve hair regeneration and grafting for medical applications.

Skin health and diseases are closely linked to metabolic processes.

This article discusses advancements in understanding how skin stem cells and fibroblasts contribute to scar formation, highlighting current and potential therapies aimed at preventing or reducing scarring, yet notes that effective treatments without significant side effects are still lacking.

This study demonstrated that cultured epidermal stem cells and skin-derived precursors can regenerate functional hair follicles and sebaceous glands in both mice and adult humans, suggesting potential for bioengineered skin substitutes.

In this study, the RADA-PRG hydrogel enhanced SKP proliferation, survival, and gene expression, effectively supporting hair follicle regeneration in mice.

Researchers created a fully functional, bioengineered skin system with hair from stem cells that successfully integrated when transplanted into mice.

This review discusses common skin appendage diseases, including their pathophysiology and treatments, but does not report new results.

This study demonstrated that a 3D air–liquid culture system using Wnt-CM-treated cells can induce hair regeneration in nude mice and may facilitate large-scale preparation for hair loss treatment.

This article reviews the role of skin appendages in wound healing and appendage regeneration, discussing cellular and molecular mechanisms but reports no new experimental findings.

This study found that a 3D Matrigel culture technique for dermal papilla cell spheroids can enhance hair follicle inductivity and induce hair-like fiber differentiation in vitro, even with high-passage cells.

This study found that human dermal papilla cells reprogram into induced pluripotent stem cells with higher efficiency than dermal fibroblasts, demonstrating their potential as an alternative source for iPS cells.

This study developed a 3D engineered hair follicle model that mimics the native hair bulb and may aid in drug screening for hair growth therapies.

This study demonstrated that PEGDA hydrogel microwells successfully supported cell survival and proliferation while mimicking hair follicle architecture in vitro, suggesting a potential tool for hair follicle engineering.

This study found that ectodermal precursor cells derived from human induced pluripotent stem cells may enhance hair follicle morphogenesis through improved epithelial-mesenchymal interactions when cocultured with dermal cells.

This study found that both human and rat dermal papilla spheroids can induce hair follicle neogenesis, but larger spheroids increase inductivity without significantly affecting hair fiber diameter.

This review discusses the role of signaling networks in sebaceous gland development and disorders, highlighting recent insights from mouse models and cell line studies, but reports no new experimental results.

This study found multipotent progenitors in sweat ducts that become unipotent after sweat gland development, highlighting distinct regenerative capabilities in adult glandular skin stem cell populations.

This study reports that hair regeneration in live mice involves spatially regulated stem cell divisions and requires mesenchymal interactions, observed in real-time using a novel non-invasive imaging method.

This study demonstrates the potential of using bioengineered follicles from adult tissue-derived stem cells for fully functional hair regeneration, illustrating possible applications in organ replacement therapy.

This article explores how extrafollicular environments and stem cell configurations influence hair and feather regeneration but provides no new experimental results.

This study reported that a three-dimensional hydrogel culture system supports the growth and maintenance of distinct dermal papilla cell types, crucial for skin reconstitution assays in neonatal mice.

This study found that TSC2-null fibroblast cells from TSC skin hamartomas can induce hair follicle formation and hamartomatous changes in keratinocytes, with active mTOR signaling observable in a mouse xenograft model.

This study successfully recreated human hair follicles in vitro, which offers a potential advancement in hair regeneration and understanding the effects of drugs on hair follicles.

In this study, researchers observed that human sebaceous glands can regenerate in a mouse model using transplanted human skin, potentially offering a tool to study sebaceous gland biology and regeneration.

This study suggests that Lrig1-positive cells form a previously unknown reservoir of interfollicular epidermal stem cells in adult mouse skin, influencing epidermal proliferation and hair follicle development.

This review discusses the reuse of molecular mechanisms involved in embryonic skin development for maintaining adult epidermal homeostasis and reports no new results.

This review summarizes fundamental concepts and recent advancements in hair follicle biology, including insights from mouse models into broader molecular and cellular processes relevant to regeneration and development.

This study presents a novel in vitro assay using human folliculoid microspheres to research hair growth, which may facilitate preclinical testing of hair growth-modulatory agents.

This study found that poly(ethylene-co-vinyl alcohol) membranes support the self-assembly of dermal papilla cells into compact spheroidal microtissues capable of inducing new hair follicles, suggesting potential for large-scale production for hair follicle regeneration.

This review explores how adult skin epithelia preserve stem cell populations for hair follicle regeneration and wound healing, but reports no new experimental results.

This study found that dermal papilla and sheath cells can induce hair follicle formation in vitro and in vivo by interacting with hair follicle epithelial cells.

This study found that bulge cells marked by CD200+ in human hair follicles show high colony-forming efficiency, suggesting successful enrichment of keratinocyte stem cells.

This study found that rat whisker follicle stem cells can be expanded in culture and transplanted to form functional hair follicles, demonstrating their multipotency and potential for regenerative medicine.

This review summarizes recent advances in understanding the molecular mechanisms of hair follicle formation and discusses potential future clinical applications for treating hair loss and skin tumors, but it reports no new clinical results.

This study found that VEGF significantly enhances perifollicular vascularization during the hair growth phase, accelerating hair regrowth and increasing follicle and hair size, while its inhibition retards hair growth.

This study demonstrated that multipotent stem cells in adult mice whisker follicles migrate to produce whisker growth, and that this process requires precise control of stem cell trafficking.

In this study, researchers found that hair follicle stem cells in mice can generate both hair follicle and epidermis cells, highlighting their potential bipotency.

This article discusses the biologic and psychosocial significance of hair, the current limitations in hair growth drugs, and anticipates future therapies based on advancing hair follicle research.