Generation of iPS Cells from Human Hair Follicle Dermal Papilla Cells

    March 2014 in “ Acta naturae
    I. A. Muchkaeva, Erdem Dashinimaev, Alexander Artyuhov, E. P. Myagkova, E. A. Vorotelyak, Yegor Yegorov, Khava S. Vishnyakova, J. E. Kravchenko, С. П. Чумаков, V. V. Terskikh, Andrei Vasiliev
    TLDR Researchers made stem cells from human hair follicle cells with higher efficiency than from skin cells.
    Researchers generated induced pluripotent stem (iPS) cells from human hair follicle dermal papilla (DP) cells using lentiviral transfection with Oct4, Sox2, Klf4, and c-Myc, and cultivated them in a medium with valproic acid at 5% oxygen. The reprogramming efficiency of DP cells was 0.03%, higher than the 0.01% efficiency in dermal fibroblasts. This study demonstrated that DP cells could be a viable alternative source for generating iPS cells.
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