In Vitro Models to Study Hair Follicle Generation

November 2017 in “ Elsevier eBooks ”

Ana Korosec, Beate M. Lichtenberger

Studysummary This review highlights recent advances in hair follicle research, noting that new 3D culture techniques have enabled formation of HF-like structures in vitro, offering potential benefits for alopecia and burn patients, but it reports no new clinical results.

Automatically generated from the study's abstract, not written by a person, and not a review of the full paper. Not medical advice or a treatment recommendation. Read the original study, and consult a qualified healthcare professional before changing treatment. Full disclaimer

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research Multifaceted Role of Hair Follicle Dermal Cells in Bioengineered Skins

33 citations , September 2016 in “British journal of dermatology/British journal of dermatology, Supplement”

This study found that human hair follicle dermal cells, specifically dermal sheath cells, can serve as an alternative and potentially superior cell source for constructing the dermal component of bioengineered skin in both in vitro and in vivo settings.

research Activation of Beta-Catenin Signaling in CD133-Positive Dermal Papilla Cells Drives Postnatal Hair Growth

26 citations , July 2016 in “PLOS ONE”

This study demonstrated that β-catenin signaling in CD133+ dermal papilla cells enhances postnatal hair growth by accelerating the proliferation and differentiation of keratinocytes in the murine hair cycle.

research Activating Beta-Catenin Signaling in CD133-Positive Dermal Papilla Cells Increases Hair Inductivity

23 citations , June 2016 in “FEBS Journal”

The study found that up-regulating β-catenin signaling in CD 133-positive dermal papilla cells enhanced their ability to promote hair follicle regeneration both in vitro and in vivo.

research Scarless Wound Healing: Finding the Right Cells and Signals

132 citations , June 2016 in “Cell and Tissue Research”

This review discusses recent advancements in scar reduction techniques, highlighting promising therapeutic targets and the role of Wnt/β-catenin signaling in promoting scarless wound healing, but reports no new clinical results.

Differential Expression Between Human Dermal Papilla Cells from Balding and Non-Balding Scalps Reveals New Candidate Genes for Androgenetic Alopecia

research Differential Expression Between Human Dermal Papilla Cells from Balding and Non-Balding Scalps Reveals New Candidate Genes for Androgenetic Alopecia

46 citations , April 2016 in “Journal of Investigative Dermatology”

This study suggests that down-regulation of vasculature-related genes in dermal papilla cells from balding scalps might contribute to the development of androgenetic alopecia.

research Epidermal β-Catenin Activation Remodels the Dermis via Paracrine Signaling to Distinct Fibroblast Lineages

115 citations , February 2016 in “Nature Communications”

The authors concluded that the dermal response to epidermal Wnt/β-catenin signaling depends on distinct fibroblast lineages, with each responding to different paracrine signals such as Hedgehog and TGF-β.

research Inhibition of β-Catenin Signaling in Dermal Fibroblasts Enhances Hair Follicle Regeneration During Wound Healing

87 citations , January 2016 in “Development”

This study suggests that the postnatal loss of hair follicle formation in wounds may be due to elevated Wnt/β-catenin activation in the dermis, affecting fibroblast function in mice.

research Fate of Prominin-1 Expressing Dermal Papilla Cells During Homeostasis, Wound Healing, and Wnt Activation

32 citations , August 2015 in “Journal of Investigative Dermatology”

In this study using a mouse model, Prom1+ dermal papilla cells were found to regulate the size of the papilla via β-catenin communication but did not aid in dermal repair.

research Human Hair Follicle Organ Culture: Theory, Application, and Perspectives

130 citations , August 2015 in “Experimental Dermatology”

This review provides a comprehensive guide to serum-free human hair follicle organ culture methods, highlighting research opportunities, standardization efforts, and potential applications without reporting new empirical findings.

research The Dynamic Duo: Niche and Stem Cell Interdependency

31 citations , May 2015 in “Stem Cell Reports”

This research discusses a novel imaging approach in live mice to study stem cell and niche interactions in tissue homeostasis and reports no new results.

research Epidermal Stem Cells and Skin Tissue Engineering in Hair Follicle Regeneration

83 citations , January 2015 in “World Journal of Stem Cells”

This review discusses current experimental approaches for regenerating human hair follicles using tissue engineering and isolated cells, but reports no successful strategies with adult cells yet found.

research Hair Follicle Dermal Stem Cells Regenerate the Dermal Sheath, Repopulate the Dermal Papilla, and Modulate Hair Type

184 citations , November 2014 in “Developmental Cell”

This study identifies a bipotent stem cell in adult hair follicles that self-renews and contributes to dermal sheath and papilla cell populations, potentially aiding hair regrowth after injury, disease, or aging.

research Controllable Production of Transplantable Adult Human High-Passage Dermal Papilla Spheroids Using 3D Matrigel Culture

46 citations , September 2014 in “Tissue engineering. Part A”

This study found that a 3D Matrigel culture technique for dermal papilla cell spheroids can enhance hair follicle inductivity and induce hair-like fiber differentiation in vitro, even with high-passage cells.

Dermal Papilla Cells Improve Wound Healing and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

research Dermal Papilla Cells Improve Wound Healing and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

68 citations , August 2014 in “Stem Cells Translational Medicine”

This study found that composite skin constructs containing dermal papilla cells promoted better skin healing and hair regeneration in nude mice, highlighting their role in tissue-engineered skin for severe injuries.

research Emerging Interactions Between Skin Stem Cells and Their Niches

426 citations , August 2014 in “Nature Medicine”

This narrative review examines the interactions between skin stem cells and their niches, finding new insights from advancements in genetic and imaging tools but reporting no new experimental results.

research Neuroendocrinology of the Hair Follicle: Principles and Clinical Perspectives

98 citations , July 2014 in “Trends in Molecular Medicine”

This article discusses the role of human hair follicles in neuroendocrinology and suggests potential new therapeutic targets by examining how neuromediators influence hair growth, pigmentation, and stem cell biology in organ cultures.

research Plasticity of Epithelial Stem Cells in Tissue Regeneration

479 citations , June 2014 in “Science”

This review discusses the plasticity of epithelial stem cells in regenerative contexts and reports no new research findings; the authors highlight potential implications for regenerative medicine and cancer.

research Defining Dermal Adipose Tissue

218 citations , May 2014 in “Experimental Dermatology”

This review explores the evolution and development of skin-associated adipose tissue and proposes new nomenclature for the cells and adipose tissue underlying the reticular dermis, without reporting clinical results.

research Novel Skin Phenotypes Revealed by a Genome-Wide Mouse Reverse Genetic Screen

50 citations , April 2014 in “Nature Communications”

This study analyzed skin from 538 knockout mouse mutants and identified 50 with epidermal phenotypes, providing valuable insights into genetic conditions and systemic effects related to skin abnormalities.

research Epidermal Wnt/β-Catenin Signaling Regulates Adipocyte Differentiation via Secretion of Adipogenic Factors

130 citations , March 2014 in “Proceedings of the National Academy of Sciences of the United States of America”

This study found that in mice, epidermal Wnt/β-catenin signaling influences adipocyte differentiation and hair growth, suggesting its critical role in synchronizing adipogenesis with the hair growth cycle.

research Stem Cell Dynamics in the Hair Follicle Niche

135 citations , December 2013 in “Seminars in Cell & Developmental Biology”

This review examines the literature on the molecular and cellular makeup of the hair follicle niche and how it influences stem cell behavior during hair regeneration, but does not provide new experimental results.

research Distinct Fibroblast Lineages Determine Dermal Architecture in Skin Development and Repair

1235 citations , December 2013 in “Nature”

This study found that skin fibroblasts in mice arise from two distinct lineages which contribute differently to dermal structure and repair, impacting hair follicle formation during wound healing.

research The Role of Hair Follicle Immune Privilege Collapse in Alopecia Areata: Status and Perspectives

79 citations , December 2013 in “Journal of Investigative Dermatology Symposium Proceedings”

This article proposes that alopecia areata may be caused by immune attacks on hair follicles after an immune privilege collapse, suggesting new therapeutic approaches targeting this immune response pattern.

research Keratin 79 Identifies a Novel Population of Migratory Epithelial Cells That Initiates Hair Canal Morphogenesis and Regeneration

60 citations , November 2013 in “Development”

This study found that the creation of hair follicle lumens in mice is driven by the outward migration of keratin 79-positive cells, suggesting a novel mechanism for generating hollow cores in hair follicles.

Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

research Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

256 citations , October 2013 in “Proceedings of the National Academy of Sciences of the United States of America”

This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.

research Dissociated Human Dermal Papilla Cells Induce Hair Follicle Neogenesis in Grafted Dermal-Epidermal Composites

51 citations , August 2013 in “Journal of Investigative Dermatology”

This study demonstrated that cultured human dermal papilla cells can induce full hair follicle formation when combined with neonatal foreskin keratinocytes in a grafted dermal-epidermal construct on mice.

research Dermal Papilla Cell Number Specifies Hair Size, Shape, and Cycling, and Its Reduction Causes Follicular Decline

211 citations , April 2013 in “Development”

In this study, researchers found that the number of dermal papilla cells in hair follicles determines hair size and shape in mice, suggesting potential avenues for addressing hair loss.

research A Pilot Clinical Study of Hair Grafting in Chronic Leg Ulcers

63 citations , October 2012 in “Wound Repair and Regeneration”

This study found that transplanting autologous scalp follicular grafts into chronic leg ulcers led to a greater reduction in ulcer size compared to non-grafted areas, suggesting a promising therapeutic alternative.

research Mutant Laboratory Mice With Abnormalities in Hair Follicle Morphogenesis, Cycling, and Structure: An Update

52 citations , October 2012 in “Journal of Dermatological Science”

This review presents updated tables of mouse mutants with hair growth abnormalities to aid in understanding the molecular mechanisms of human hair disorders, but reports no new clinical results.

research Mesenchymal–Epithelial Interactions During Hair Follicle Morphogenesis and Cycling

300 citations , August 2012 in “Seminars in Cell & Developmental Biology”

This review discusses mesenchymal–epithelial interactions in hair follicle development and cycling, highlighting recent insights without reporting new experimental results.

research Morphogenesis of Chimeric Hair Follicles in Engineered Skin Substitutes With Human Keratinocytes and Murine Dermal Papilla Cells

46 citations , August 2012 in “Experimental Dermatology”

In this study, engineered skin substitutes containing murine dermal papilla cells developed hair follicle-like structures when grafted onto mice, suggesting a model for generating chimeric hair.

research Shh Maintains Dermal Papilla Identity and Hair Morphogenesis via a Noggin–Shh Regulatory Loop

138 citations , June 2012 in “Genes & Development”

This study found that dermal Shh signaling regulates specific dermal papilla signatures essential for maintaining hair follicle development, suggesting that the Shh-Noggin signaling loop is crucial for hair morphogenesis.

research Reprogramming of Human Hair Follicle Dermal Papilla Cells into Induced Pluripotent Stem Cells

26 citations , February 2012 in “Journal of Investigative Dermatology”

This study found that dermal papilla cells from human hair follicles can be reprogrammed into induced pluripotent stem cells, but their efficiency is similar to that of fibroblasts, unlike in mice.

Hair Follicle Stem Cell Differentiation Is Inhibited Through Cross-Talk Between Wnt/β-Catenin and Androgen Signaling in Dermal Papilla Cells From Patients With Androgenetic Alopecia

research Hair Follicle Stem Cell Differentiation Is Inhibited Through Cross-Talk Between Wnt/β-Catenin and Androgen Signaling in Dermal Papilla Cells From Patients With Androgenetic Alopecia

95 citations , January 2012 in “British Journal of Dermatology”

This study found that androgens inhibit hair follicle stem cell differentiation by disrupting the Wnt signalling pathway in dermal papilla cells, but Wnt activation can restore differentiation.

research Reprogramming Adult Dermis to a Neonatal State Through Epidermal Activation of Beta-Catenin

128 citations , October 2011 in “Development”

This study found that adult mouse dermis can be reprogrammed by epidermal cues to resemble neonatal dermis, with changes in fibroblast proliferation and extracellular matrix composition.

Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

research Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

75 citations , August 2011 in “Journal of Investigative Dermatology”

This study found that cultured human dermal papilla cells formed into three-dimensional spheres have an increased ability to induce hair follicles from mouse epidermal cells compared to two-dimensional cultures.

research Melanocyte Stem Cells: A Melanocyte Reservoir in Hair Follicles for Hair and Skin Pigmentation

240 citations , April 2011 in “Pigment Cell & Melanoma Research”

This review discusses the identification and characteristics of melanocyte stem cells, emphasizing their role in hair pigmentation, greying, and skin repigmentation, but reports no new research findings.

research The Basement Membrane Of Hair Follicle Stem Cells Is A Muscle Cell Niche

294 citations , February 2011 in “Cell”

Nephronectin helps attach muscle cells to hair follicles.

research De Novo Formation and Ultra-Structural Characterization of a Fiber-Producing Human Hair Follicle Equivalent In Vitro

44 citations , January 2011 in “Journal of biotechnology”

This study successfully recreated human hair follicles in vitro, which offers a potential advancement in hair regeneration and understanding the effects of drugs on hair follicles.

Bald Scalp in Men with Androgenetic Alopecia Retains Hair Follicle Stem Cells but Lacks CD200-Rich and CD34-Positive Hair Follicle Progenitor Cells

research Bald Scalp in Men with Androgenetic Alopecia Retains Hair Follicle Stem Cells but Lacks CD200-Rich and CD34-Positive Hair Follicle Progenitor Cells

235 citations , January 2011 in “Journal of Clinical Investigation”

This study found that androgenetic alopecia may involve a defect in the conversion of hair follicle stem cells to progenitor cells, as demonstrated by a reduced presence of progenitor cells in bald scalp samples.

research Beta-Catenin Activity in the Dermal Papilla Regulates Morphogenesis and Regeneration of Hair

314 citations , April 2010 in “Developmental Cell”

This study found that in mice, inactivating beta-catenin in the dermal papilla reduces hair follicle progenitor proliferation and disrupts the hair cycle.

research Dermal Papilla Cells Induce Keratinocyte Tubulogenesis in Culture

28 citations , March 2010 in “Histochemistry and cell biology”

Skin cells can help create early hair-like structures in lab cultures.

research Review of Hair Follicle Dermal Cells

321 citations , December 2009 in “Journal of Dermatological Science”

This review discusses the role of dermal cells in hair follicle regeneration and morphogenesis and highlights their potential therapeutic implications, but it reports no new empirical findings.

research Hair Follicle Neogenesis Induced by Cultured Human Scalp Dermal Papilla Cells

63 citations , September 2009 in “Regenerative Medicine”

This study developed a method for expanding human dermal papilla cells in vitro while maintaining their ability to induce hair growth, advancing the potential for follicular cell implantation as a treatment for hair loss.

research The Mesenchymal Component of Hair Follicle Neogenesis: Background, Methods, and Molecular Characterization

131 citations , July 2009 in “Experimental Dermatology”

This review discusses the development and advances in studying trichogenic dermal cells for hair follicle morphogenesis, summarizing methods, bioassays, and molecular markers, but reports no new research findings.

research Sox2-Positive Dermal Papilla Cells Specify Hair Follicle Type in Mammalian Epidermis

338 citations , July 2009 in “Development”

This study demonstrates that Sox2-positive dermal papilla cells specify particular hair follicle types by showing heterogeneity in gene expression and pathway activation in these cells.

research The Hair Follicle as a Dynamic Mini-Organ

759 citations , February 2009 in “Current Biology”

This review summarizes fundamental concepts and recent advancements in hair follicle biology, including insights from mouse models into broader molecular and cellular processes relevant to regeneration and development.

research A Human Folliculoid Microsphere Assay for Exploring Epithelial-Mesenchymal Interactions in the Human Hair Follicle

71 citations , October 2008 in “The journal of investigative dermatology/Journal of investigative dermatology”

This study presents a novel in vitro assay using human folliculoid microspheres to research hair growth, which may facilitate preclinical testing of hair growth-modulatory agents.

research More Than One Way to Skin

218 citations , April 2008 in “Genes & Development”

This research discusses how epithelial stem cells in the skin maintain normal homeostasis, contribute to wound repair, and highlights the importance of these processes, as defects can lead to skin diseases including cancers, but provides no new experimental results.

research Building Epithelial Tissues From Skin Stem Cells

84 citations , January 2008 in “Cold Spring Harbor Symposia on Quantitative Biology”

This article reviews recent advancements in understanding skin stem cells and their roles in maintaining epidermal homeostasis and repairing wounds, without presenting new experimental findings.

research Wnt-Dependent De Novo Hair Follicle Regeneration in Adult Mouse Skin After Wounding

829 citations , May 2007 in “Nature”

Hair follicles can regrow in wounded adult mouse skin using a process like embryo development.

research Hair Follicle Regeneration Using Grafted Rodent and Human Cells

96 citations , April 2007 in “Journal of Investigative Dermatology”

In this study, co-grafting human keratinocytes with murine dermal papilla-enriched cells produced hair follicle-like structures, but without regular hair formation, suggesting anomalous folliculogenesis.

research Scratching the Surface of Skin Development

788 citations , February 2007 in “Nature”

This review explores how adult skin epithelia preserve stem cell populations for hair follicle regeneration and wound healing, but reports no new experimental results.

research The Hair Cycle

375 citations , February 2006 in “Journal of Cell Science”

This article reviews the stages and regulation of the hair cycle, highlighting molecular regulators involved, but it does not present new research findings.

research Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle

417 citations , September 2005 in “PLoS biology”

This study developed molecular signatures for dermal papilla cells and their niche, uncovering novel signaling regulators and genes linked to hair disorders, which may inform future hair development research.

research Organogenesis From Dissociated Cells: Generation of Mature Cycling Hair Follicles From Skin-Derived Cells

205 citations , April 2005 in “Journal of Investigative Dermatology”

This study describes a system for generating hair follicles from dissociated cells in mice, suggesting that successful development requires a foundational epithelial platform.

research Molecular Principles of Hair Follicle Induction and Morphogenesis

479 citations , January 2005 in “BioEssays”

This review introduces the morphological and molecular principles of hair follicle development but reports no new experimental results, focusing on recent insights and forming a working hypothesis.

research Dermal Papilla-Induced Hair Differentiation of Adult Epithelial Stem Cells from Human Skin

90 citations , August 2004 in “Physiological Genomics”

This study found that coculturing human hair follicle keratinocyte stem cells with dermal papilla cells increased expression of the hair-specific keratin 6hf gene, which requires β-catenin/lef-1 signaling for hair differentiation.

research Molecular Biology of Hair Morphogenesis: Development and Cycling

155 citations , August 2003 in “Journal Of Experimental Zoology Part B: Molecular And Developmental Evolution”

This review discusses the conserved molecular mechanisms controlling hair follicle development and cycling and suggests they may also apply to other ectodermal derivatives, like teeth and feathers, but it reports no new results.

research Animal and In Vitro Models for the Study of Hair Follicles

45 citations , June 2003 in “Journal of Investigative Dermatology Symposium Proceedings”

This review discusses various in vitro and in vivo models for studying hair follicle function, noting their potential in developing new treatments for hair disorders but reports no new results.

research Molecular Mechanisms Regulating Hair Follicle Development

854 citations , February 2002 in “The journal of investigative dermatology/Journal of investigative dermatology”

This review summarizes recent advances in understanding the molecular mechanisms of hair follicle formation and discusses potential future clinical applications for treating hair loss and skin tumors, but it reports no new clinical results.

research Trans-Species Hair Growth Induction by Human Hair Follicle Dermal Papillae

66 citations , August 2001 in “Experimental Dermatology”

This study demonstrated that intact adult human dermal papillae can induce hair growth when implanted into mouse hair follicles, highlighting their potential for advancing hair follicle biology and therapeutic approaches.

research The Chemotactic Effect of a Dermal Papilla Cell-Derived Factor on Outer Root Sheath Cells

47 citations , April 2001 in “Journal of Dermatological Science”

This study found that insulin-like growth factor-I and hepatocyte growth factor increased the chemotactic migration of human hair outer root sheath cells, suggesting these factors have both growth stimulatory and chemotactic effects.

research The Biology of Hair Follicles

1113 citations , August 1999 in “The New England Journal of Medicine”

This article discusses the biologic and psychosocial significance of hair, the current limitations in hair growth drugs, and anticipates future therapies based on advancing hair follicle research.

research Whole Hair Follicle Culture

57 citations , October 1996 in “Dermatologic clinics”

This study found that using hyaluronic acid microneedles to deliver minoxidil significantly reduced hair loss in chemotherapy-induced alopecia mice, with only 10% of the dose used in topical applications.

research Hair Matrix Germinative Epidermal Cells Confer Follicle-Inducing Capabilities on Dermal Sheath and High Passage Papilla Cells

61 citations , October 1996 in “Development”

This study found that combining adult germinative epidermal cells with non-inductive dermal cells can stimulate hair follicle neogenesis, effectively altering the cells' status to enhance induction.

research Successful Treatment of Chronic Leg Ulcers with Epidermal Equivalents Generated from Cultured Autologous Outer Root Sheath Cells

99 citations , July 1996 in “Journal of Investigative Dermatology”

Using patient's own hair cells can effectively heal chronic leg ulcers.

research Human Hair Growth In Vitro: A Model for the Study of Hair Follicle Biology

133 citations , July 1994 in “Journal of Dermatological Science”

In this study, the researchers developed an in vitro model that maintains human hair follicles to grow at in vivo rates and observed that factors like EGF and IGF-I influence hair follicle growth and catagen entry.

research Soluble Factors From Human Hair Papilla Cells And Dermal Fibroblasts Dramatically Increase The Clonal Growth Of Outer Root Sheath Cells

62 citations , June 1993 in “Archives of Dermatological Research”

research Rat Hair Follicle Growth In Vitro

58 citations , December 1992 in “British Journal of Dermatology”

In this study, insulin-like growth factor-1 did not impact hair follicle elongation in rat pelage hair follicles, while transforming growth factor-beta 1 inhibited both elongation and thymidine uptake.

research Induction of Follicle Formation and Hair Growth by Vibrissa Dermal Papillae Implanted into Rat Ear Wounds: Vibrissa-Type Fibres Are Specified

125 citations , August 1992 in “Development”

This study found that implanting isolated adult rat dermal papillae into ear cuts led to the emergence of unusually large vibrissa-type hair follicles, demonstrating their capacity to induce specific hair fiber characteristics.

research Cellular and Extracellular Involvement in the Regeneration of the Rat Lower Vibrissa Follicle

39 citations , April 1992 in “Development”

In this study, researchers detailed how hair follicles regenerate after microsurgical amputation, observing a wound reaction involving dynamic dermal-epidermal interactions and changes in extracellular matrix components.

research An In Vitro Model for the Study of Human Hair Growth

13 citations , December 1991 in “Annals of the New York Academy of Sciences”

This study observed that human anagen hair follicles can grow in vitro when maintained serum-free, with possible implications for understanding hair follicle growth mechanisms.

research Human Hair Growth In Vitro

385 citations , November 1990 in “Journal of Cell Science”

This study successfully grew and maintained human hair follicles in vitro, noting that TGF-β1 negatively affects growth, while EGF replicates hair-removal effects seen in animals.