Effects of Photobiomodulation on Human Hair Dermal Papilla Cells with Various Light Modes and Parameters

    Yi Ren, Angze Li, Xiaojing Miao, Longfei Huo, Haokuan Qin, Hui Jiang, Muqing Liu
    TLDR Pulsed wave red LED light is more effective than continuous wave light for promoting hair regrowth.
    This study examines the impact of photobiomodulation (PBM) on human hair dermal papilla cells using 660 nm red LED light, finding that pulsed wave (PW) PBM is more effective than continuous wave (CW) PBM in promoting hair regrowth. PW PBM enhances cell viability, proliferation, and migration, with optimal parameters identified as a peak irradiance of 10 mW/cm², duty cycle of 80%, frequency of 500 Hz, and dose of 8.8 J/cm². The study reveals that PW PBM activates the Wnt/β-Catenin signaling pathway and inhibits Transforming Growth Factor signaling. These results suggest that PW PBM could be a promising non-invasive treatment for androgenetic alopecia, providing a safer alternative to traditional drugs like minoxidil and finasteride, which have significant side effects.
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