Two-Cell Assemblage Assay: A Simple In Vitro Method for Screening Hair Growth-Promoting Compounds

    Sunhyae Jang, Jungyoon Ohn, Byungjun Kang … Ohsang Kwon
    Studysummary This study introduced a simple and reliable in vitro assay capable of large-scale simultaneous screening of hair growth-promoting compounds using a 3D co-culture system of human dermal papilla and outer root sheath cells.
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    Research cited in this study 39

    1. Functional Complexity of Hair Follicle Stem Cell Niche and Therapeutic Targeting of Niche Dysfunction for Hair Regeneration Journal of Biomedical Science · 2020
    2. Shikimic Acid, a Mannose Bioisostere, Promotes Hair Growth with the Induction of Anagen Hair Cycle. Scientific Reports · 2019
    3. Keratinocytes Maintain Compartmentalization Between Dermal Papilla and Fibroblasts in 3D Heterotypic Tri-Cultures Cell Proliferation · 2019
    4. Preparation of Hair Beads and Hair Follicle Germs for Regenerative Medicine Biomaterials · 2019
    5. Evaluating Hair Growth Promoting Effects of Candidate Substances: A Review of Research Methods Journal of Dermatological Science · 2019
    6. Self-Organizing Hair Peg-Like Structures From Dissociated Skin Progenitor Cells: New Insights for Human Hair Follicle Organoid Engineering and Turing Patterning in an Asymmetric Morphogenetic Field Experimental Dermatology · 2019
    7. Review of Hair Follicle Dermal Papilla Cells as In Vitro Screening Model for Hair Growth International Journal of Cosmetic Science · 2018
    8. External Light Activates Hair Follicle Stem Cells Through Eyes Via an ipRGC–SCN–Sympathetic Neural Pathway Proceedings of the National Academy of Sciences of the United States of America · 2018
    9. Establishment of an In Vitro Organoid Model of Dermal Papilla of Human Hair Follicle Journal of Cellular Physiology · 2018
    10. Getting to the Core of the Dermal Papilla Journal of Investigative Dermatology · 2017
    11. Self-Organization Process in Newborn Skin Organoid Formation Inspires Strategy to Restore Hair Regeneration of Adult Cells Proceedings of the National Academy of Sciences of the United States of America · 2017
    12. Next Generation Human Skin Constructs as Advanced Tools for Drug Development Experimental Biology and Medicine · 2017
    13. Methods for the Isolation and 3D Culture of Dermal Papilla Cells from Human Hair Follicles Experimental Dermatology · 2017
    14. Stress-Induced Premature Senescence of Dermal Papilla Cells Compromises Hair Follicle Epithelial-Mesenchymal Interaction Journal of Dermatological Science · 2017
    15. Aging, Alopecia, and Stem Cells Science · 2016
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    18. A Two-Step Culture Method for Efficient Production of Trichogenic Keratinocytes Tissue Engineering Part C-methods · 2015
    19. Topical Valproic Acid Increases Hair Count in Male Patients with Androgenetic Alopecia: A Randomized, Comparative, Clinical Feasibility Study Using Phototrichogram Analysis Journal of Dermatology · 2014
    20. Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth Proceedings of the National Academy of Sciences of the United States of America · 2013
    21. Follicular Dermal Papilla Structures by Organization of Epithelial and Mesenchymal Cells in Interfacial Polyelectrolyte Complex Fibers Biomaterials · 2013
    22. Scalable Production of Controllable Dermal Papilla Spheroids on PVA Surfaces and the Effects of Spheroid Size on Hair Follicle Regeneration Biomaterials · 2012
    23. Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay Journal of Investigative Dermatology · 2011
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    26. A Cell-Based System for Screening Hair Growth-Promoting Agents Archives of Dermatological Research · 2009
    27. A Human Folliculoid Microsphere Assay for Exploring Epithelial-Mesenchymal Interactions in the Human Hair Follicle ˜The œjournal of investigative dermatology/Journal of investigative dermatology · 2008
    28. Adenosine Stimulates Fibroblast Growth Factor-7 Gene Expression Via Adenosine A2b Receptor Signaling in Dermal Papilla Cells ˜The œjournal of investigative dermatology/Journal of investigative dermatology · 2007
    29. PDGF Isoforms Induce and Maintain Anagen Phase of Murine Hair Follicles Journal of Dermatological Science · 2006
    30. Modulation of Hair Growth with Small Molecule Agonists of the Hedgehog Signaling Pathway Journal of Investigative Dermatology · 2005
    31. Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle PLoS biology · 2005
    32. Minoxidil: Mechanisms of Action on Hair Growth British Journal of Dermatology · 2004
    33. Simple and Rapid Method to Isolate and Culture Follicular Papillae from Human Scalp Hair Follicles Experimental Dermatology · 2002
    34. Control of Hair Growth and Follicle Size by VEGF-Mediated Angiogenesis Journal of Clinical Investigation · 2001
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    38. The Psychological Effects of Androgenetic Alopecia in Men Journal of The American Academy of Dermatology · 1992
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