A Two-Step Culture Method for Efficient Production of Trichogenic Keratinocytes

    Chih-Chieh Chan, Shih-Kang Fan, Wei-Hung Wang, Yifen Mu, Sung-Jan Lin
    Image of study
    TLDR A two-step method was created in 2015 to make more cells that help with hair growth, but they need to be combined with other cells for 4 days to actually form new hair.
    In 2015, researchers developed a two-step culture method to efficiently produce trichogenic keratinocytes, cells vital for hair growth. Initially, keratinocytes were cultivated in a low calcium concentration, then transferred to a high calcium concentration, resulting in a higher yield of these cells. The study found that keratinocytes could be expanded for over 100 passages without losing their proliferative potential, but they were unable to form new hair follicles (HFs) unless cocultured with HF dermal papilla (DP) cells for 4 days. This coculture enhanced Wnt/β-catenin signaling, crucial for HF development, and upregulated the expression of HF-specific genes. This method could potentially offer a more effective approach for treating hair loss conditions such as alopecia.
    Discuss this study in the Community →

    Research cited in this study

    18 / 18 results

    Related Community Posts Join

    6 / 7 results

      community New and Interesting HairLoss Studies/Papers/Reviews

      in Treatment  42 upvotes 4 years ago
      Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

      community [AMA] 5-Month Progress Report: Dutasteride and Oral Minoxidil Results.

      in Minoxidil  73 upvotes 3 years ago
      Naepo's 5-month progress report detailing their journey of treating male pattern baldness with dutasteride and oral minoxidil, which has already resulted in an encouraging improvement in hair density. Others have responded with appreciation for the thorough report and asked further questions about Naepo's educational background.

      community My experience with Trichogenics

      in Progress Pictures  6 upvotes 1 year ago
      A user shared their positive experience with Trichogenics for hair restoration, highlighting their unique zigzag hairline technique and personalized attention. They had 1700 follicles transplanted and praised the natural results.

      community TrichoGene minoxidil response test?

      in Minoxidil 1 year ago
      The conversation is about the legitimacy and affordability of ordering a Minoxidil response test from TrichoGene, an India-based company, as an alternative to Daniel Alain. The user is seeking feedback on whether TrichoGene is a reliable option.

    Related Research

    5 / 5 results