Dermal Papilla Cell-Derived Exosomes Regulate Hair Follicle Stem Cell Proliferation via LEF1

February 2023 in “ International Journal of Molecular Sciences ”

Jiali Li, Bohao Zhao, Shuyu Yao, Yingying Dai, Xiyu Zhang, Naisu Yang, Zhiyuan Bao, Jiawei Cai, Yang Chen, Xinsheng Wu

dermal papilla cells exosomes hair follicle stem cells HFSC LEF1 hair follicle development cell viability apoptosis RNA sequencing differentially expressed genes gene overexpression gene knockdown DPC-Exos cell survival cell death gene expression analysis

Studysummary This study found that exosomes derived from dermal papilla cells can enhance the proliferation of hair follicle stem cells by stimulating the LEF1 pathway, offering insights into hair follicle growth regulation.

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The study investigated the role of exosomes derived from dermal papilla cells (DPC-Exos) in hair follicle stem cell (HFSC) proliferation. It was found that DPC-Exos increased cell viability and reduced apoptosis in HFSCs. RNA sequencing identified 3702 differentially expressed genes in DPC-Exos-treated HFSCs, including LEF1, a gene associated with hair follicle development. Overexpression of LEF1 increased the expression of hair follicle development-related genes and proteins, enhanced HFSC proliferation, and reduced HFSC apoptosis. Conversely, knockdown of LEF1 reversed these effects. The study concluded that DPC-Exos can regulate HFSC proliferation by stimulating LEF1, providing new insights into hair follicle growth and development regulatory mechanisms.

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