Mesenchymal Stromal Cell-Conditioned Medium for Skin Diseases: A Systematic Review

This review article summarizes the potential role of advanced medical therapies—using genes, cells, and/or tissue engineering—in treating various types of alopecia, by examining clinical research, basic studies, and ongoing trials.

In this study, human ADSC-CM treatment following nonablative fractional laser significantly increased hair density and volume in patients with androgenetic alopecia without any reported adverse effects.

In this study using a mouse model, ECM/SVF-CM, derived from adipose-derived stem cells, was observed to stimulate hair growth more effectively than SVF-CM by promoting cell proliferation, neovascularization, and anagen induction, which may support its potential as an improved treatment for hair loss.

This study found that 5% primed conditioned medium from human umbilical cord blood-derived mesenchymal stromal cells significantly improved hair density, thickness, and growth rate in patients with androgenetic alopecia.

This study reports that mesenchymal stem cell-conditioned medium promoted dermal cell migration and proliferation, enhancing wound healing but not direct hair growth, in vitro and in adult mouse models.

This study found that preconditioning adipose-derived stem cells with HB-EGF enhanced their ability to promote hair growth in vivo by increasing their motility, paracrine effects, and survival.

This study found that adipose-derived stem cell-conditioned media improved skin flap survival following ischemia/reperfusion injury in mice by increasing cell proliferation and the number of hair follicles, mediated by IL-6.

This study found that intradermal administration of adipose-derived stem cell–conditioned medium significantly increased hair density and dermal thickness, suggesting potential regenerative effects on both hair follicles and the interfollicular scalp in patients with alopecia.

This study suggests that the secretome from amniotic fluid-derived mesenchymal stem cells overexpressing Nanog could stimulate hair growth and increase follicle density, potentially aiding in alopecia treatment.

This study found that conditioned media from dental pulp stem cells stimulated significantly faster hair growth in mice, suggesting potential as a treatment for alopecia.

The researchers reported that they predicted associations between certain components of MSC-conditioned medium and its ability to stimulate cell migration in vitro, identifying factors affecting its biological activity.

This review discusses current uses and advancements in mesenchymal stem cell-derived conditioned media as a potential regenerative therapy but does not present new clinical findings.

This study found that Wnt7a-containing conditioned medium from UC-MSCs can accelerate wound healing and promote hair follicle regeneration by enhancing specific cellular functions in the wound microenvironment.

In this study, human dermal stem/progenitor cell-derived conditioned medium was found to promote hair regrowth in mice, possibly through effects on the Wnt/β-catenin pathway.

This study found that human mesenchymal stromal cells derived from hair follicle dermal sheath showed promising growth and wound-healing properties in vitro and in diabetic mice, suggesting potential for therapeutic use.

This retrospective study found that adipose-derived stem cell-conditioned media increased hair density and thickness in women with female pattern hair loss over 12 weeks, with no severe adverse effects reported.

This study found that keratinocyte stem/progenitor cell-conditioned medium significantly enhanced hair growth in mice and increased the proliferation of human hair follicle cells.

This study found that overexpression of Wnt1a in bone marrow mesenchymal stem cells enhanced mouse hair follicle regeneration and promoted hair cycling.

This review discusses various methods for obtaining stem cell-derived conditioned media and its applications in disease treatment, finding generally positive results but highlighting the need for standardized production methods.

This study found that stem cells from the human umbilical cord's Wharton's jelly may significantly enhance healing in excisional and diabetic wounds compared to controls.

Researchers made a cell line that grows quickly and can help with hair growth research.

This study found that subcutaneous administration of conditioned medium from adipose-derived stem cells promoted hair growth in mice, and hypoxia enhanced this effect by altering growth factor secretion.