Human Wharton's Jelly Stem Cells and Their Conditioned Medium Enhance Healing of Excisional and Diabetic Wounds

The study evaluated the effectiveness of human Wharton's jelly stem cells (hWJSCs) and their conditioned medium (hWJSC-CM) in enhancing the healing of excisional and diabetic wounds. hWJSCs, derived from the human umbilical cord, were found to be non-controversial, hypo-immunogenic, and non-tumorigenic, with the ability to differentiate into keratinocytes and secrete molecules crucial for tissue repair. In vitro assays showed that hWJSC-CM significantly improved fibroblast migration and increased levels of collagen, elastin, and fibronectin compared to controls. In vivo, both GFP-labeled hWJSCs and hWJSC-CM significantly accelerated wound healing in murine models, with treated wounds exhibiting reepithelialization, increased vascularity, cellular density, and more sebaceous glands and hair follicles. The study concluded that hWJSCs enhance wound healing through differentiation and molecular secretion, demonstrating potential as a therapeutic option for wound management.