Differentiation Genes Governed by DNA Methylation During Hair Follicle Morphogenesis in Cashmere Goats

    Shanhe Wang, Fang Li, Jinwang Liu, Yuelang Zhang, Yujie Zheng, Wei Ge, Lei Qu, Xin Wang
    TLDR DNA methylation controls hair follicle gene expression in cashmere goats.
    The study investigated the role of DNA methylation in hair follicle morphogenesis in cashmere goats, using RNA-seq and Whole-genome bisulfite sequencing on embryonic skin tissues. It was found that DNA methylation negatively correlated with the expression of hair follicle differentiation genes and transcription factors, which were repressed during the hair follicle induction stage and expressed during the differentiation stage. The Wnt signaling pathway and transcription factors like Hoxc13, Sox9, and Gata3 were crucial in this process. Additionally, specific long non-coding RNAs (lncRNAs) were identified as potentially influencing DNA methylation of target genes, such as lncRNA XR_001918556 affecting Gata3. This research enhanced the understanding of the molecular mechanisms governing hair morphogenesis.
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