Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells

January 2017 in “ Methods in molecular biology ”

Kyosuke Asakawa, Koh-ei Toyoshima, Takashi Tsuji

hair follicle regeneration stem cells epithelial cells mesenchymal cells dorsal skin vibrissae follicle cells enzymatic reactions centrifugation

TLDR Stem cells rearrangement regenerates functional hair follicles, potentially treating hair loss.

The paper describes a method for functional hair follicle regeneration through the rearrangement of stem cells. The study involves the preparation of single epithelial and mesenchymal cells from the dorsal skin of embryonic mice, as well as the preparation of adult mouse vibrissae follicle cells. The process involves enzymatic reactions and centrifugation to isolate the necessary cells. The study provides detailed instructions for the preparation of these cells and their subsequent use in hair follicle regeneration. In 2012, researchers successfully regenerated fully functional hair follicles in mice by rearranging stem cells and their niches. This method could potentially be used for hair loss treatment in humans.

View this study on link.springer.com →

Discuss this study in the Community →

Research cited in this study

13 / 13 results

research Dermal Papilla Cell Number Specifies Hair Size, Shape, and Cycling, and Its Reduction Causes Follicular Decline

211 citations , April 2013 in “Development”

More dermal papilla cells in hair follicles lead to larger, healthier hair, while fewer cells cause hair thinning and loss.

research Hair Organ Regeneration Via Bioengineered Hair Follicular Unit Transplantation

66 citations , May 2012 in “Scientific Reports”

Scientists successfully created and transplanted bioengineered hair follicles that function like natural ones, suggesting a new treatment for hair loss.

research Fully Functional Hair Follicle Regeneration Through the Rearrangement of Stem Cells and Their Niches

179 citations , April 2012 in “Nature Communications”

Regenerated fully functional hair follicles using stem cells, with potential for hair regrowth therapy.

research SKPs Derive from Hair Follicle Precursors and Exhibit Properties of Adult Dermal Stem Cells

330 citations , December 2009 in “Cell stem cell”

SKPs are similar to adult skin stem cells and could help in skin repair and hair growth.

research The Hair Follicle as a Dynamic Mini-Organ

759 citations , February 2009 in “Current Biology”

Hair follicles are complex, dynamic mini-organs that help us understand cell growth, death, migration, and differentiation, as well as tissue regeneration and tumor biology.

research Lgr5 Marks Cycling, Yet Long-Lived, Hair Follicle Stem Cells

835 citations , October 2008 in “Nature Genetics”

Lgr5 is a marker for active, long-lasting stem cells in mouse hair follicles.

research Isolation and Short-Term Culture of Primary Keratinocytes, Hair Follicle Populations, and Dermal Cells from Newborn Mice and Keratinocytes from Adult Mice for In Vitro Analysis and Grafting to Immunodeficient Mice

427 citations , April 2008 in “Nature Protocols”

research Wnt-Dependent De Novo Hair Follicle Regeneration in Adult Mouse Skin After Wounding

829 citations , May 2007 in “Nature”

Hair follicles can regrow in wounded adult mouse skin using a process like embryo development.

research Bioengineering the Hair Follicle

26 citations , January 2007 in “Organogenesis”

Bioengineering can potentially treat hair loss by regenerating hair follicles and cloning hair, but the process is complex and needs more research.

research Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle

417 citations , September 2005 in “PLoS biology”

Understanding gene expression in hair follicles can reveal insights into hair growth and disorders.

research Hair Follicle Dermal Cells Differentiate Into Adipogenic and Osteogenic Lineages

268 citations , December 2003 in “Experimental Dermatology”

Hair follicle cells can become fat and bone cells.

Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

research Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

208 citations , December 2003 in “Journal of Investigative Dermatology”

Certain cells from hair follicles can create new hair and contribute to hair growth when implanted in mice.

research Morphogenesis and Renewal of Hair Follicles from Adult Multipotent Stem Cells

949 citations , January 2001 in “Cell”

Adult mouse skin contains stem cells that can create new hair, skin, and oil glands.

Related Community Posts Join

6 / 1000+ results

Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

community Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

in Research 865 upvotes 1 year ago

The treatment for androgenetic alopecia involves using finasteride and minoxidil with intense exercise and cold exposure to boost metabolism and reduce androgenic effects, potentially leading to hair regrowth. This approach may activate biological pathways for improved hair and overall health.

community UPDATE: The theory that explains everything. Please help me make this big!

in Research/Science 441 upvotes 4 years ago

Hair loss theory involves 3alpha-hydroxysteroid reductase (3AHD) converting DHT to androstenol. Discussion explores potential treatments and encourages more research.

community If You Have DUPA, PLEASE READ THIS: Everyone Should Be Scalp Biopsied

in Research 830 upvotes 1 year ago

Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.

community Microneedling and aloe vera to recruit stem cells.

in Chat 11 upvotes 1 year ago

Microneedling combined with aloe vera may stimulate dormant stem cells in hair loss areas. Minoxidil is also suggested for better results.

community HairClone developing cell replacement treatments for Hair Loss

6 upvotes 6 years ago

HairClone is developing cell replacement treatments to rejuvenate and generate hair follicles, and has launched a crowdfunding campaign. A user expressed skepticism about the need for crowdfunding.

community How Does Exosome Therapy Work for Hair Loss?

in Research/Science 5 upvotes 2 years ago

Exosome hair therapy involves injecting stem cell-derived exosomes into the scalp to boost hair follicle regeneration and repair. Exosomes, which are not stem cells but products of them, contain bioactive chemicals and specific growth factors that promote new blood vessel formation, increase cell proliferation, reduce inflammation, and improve tissue repair, all crucial for hair health.

Related Research

2 / 2 results

research Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells

8 citations , January 2017 in “Methods in molecular biology”

Stem cells rearrangement regenerates functional hair follicles, potentially treating hair loss.

research Hair Follicle Regeneration In Vitro

December 2016 in “Paleontological Journal”

Hair growth can be induced by transplanting certain cells, but these cells lose their properties during culturing. The best cell interaction happens in a liquid medium under gravity, and using collagen doesn't help. Future research could focus on using growth factors to stimulate these cells.