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GlossaryCell Culture (tissue culture)

process of growing cells in controlled laboratory conditions

Cell culture, also known as cell cultivation or in vitro cell culture, is a laboratory technique where cells are grown under controlled conditions, typically outside their natural environment. This process involves providing the cells with the necessary nutrients, temperature, and gases to proliferate, allowing researchers to study cellular behaviors, test drug effects, and produce biological compounds in a controlled setting.

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A Model System for Long-Term Serum-Free Suspension Organ Culture of Human Fetal Tissues: Experiments on Digits and Skin from Multiple Body Regions

research A Model System for Long-Term Serum-Free Suspension Organ Culture of Human Fetal Tissues: Experiments on Digits and Skin from Multiple Body Regions

17 citations, January 1997 in “Cell and Tissue Research”

Scientists developed a method to grow human fetal skin and digits in a lab for 3-4 weeks, which could help study skin features and understand genetic interactions in tissue formation.

research Establishment of an Efficient Primary Culture System for Human Hair Follicle Stem Cells Using the Rho-Associated Protein Kinase Inhibitor Y-27632

16 citations, March 2021 in “Frontiers in cell and developmental biology”

A new method using Y-27632 improves the growth and quality of human hair follicle stem cells for tissue engineering and therapy.

research Skin And Hair On-A-Chip: In Vitro Skin Models Versus Ex Vivo Tissue Maintenance With Dynamic Perfusion

208 citations, January 2013 in “Lab on a Chip”

The Multi-Organ-Chip improves the growth and quality of skin and hair in the lab, potentially replacing animal testing.

research Screening of New Agents for Hair Growth and Their Mechanisms of Action

June 1996 in “Journal of Dermatological Science”

Two new compounds were found that could promote hair growth as well or better than minoxidil.

research Generation And Characterization Of Hair-Bearing Skin Organoids From Human Pluripotent Stem Cells

39 citations, March 2022 in “Nature Protocols”

Scientists created hair-growing skin models from stem cells, which could help treat hair loss and skin diseases.

research Tissue Engineering of the Reproductive System

1 citations, December 2010 in “Elsevier eBooks”

Cell transplantation faces challenges in genitourinary reconstruction, but alternative tissue sources and microencapsulation show promise.

research Scalable and High-Throughput Production of an Injectable Platelet-Rich Plasma (PRP)/Cell-Laden Microcarrier/Hydrogel Composite System for Hair Follicle Tissue Engineering

November 2022 in “Journal of Nanobiotechnology”

The developed system could effectively treat hair loss and promote hair growth.

research An Appraisal of Laboratory Models of Androgenetic Alopecia: A Systematic Review

1 citations, March 2021 in “Skin health and disease”

Better hair loss models needed for research.

research Reorganization of Hair Follicles in Human Skin Organ Culture Induced by Cultured Human Follicle-Derived Cells

56 citations, July 2005 in “Experimental Dermatology”

Injected human hair follicle cells can create new, small hair follicles in skin cultures.

research Human Hair Keratin Hydrogel: Fabrication, Characterization, and Application

January 2014

Keratin hydrogels from human hair show promise for tissue engineering and regenerative medicine.

research Cells, Growth Factors and Biomaterials Used in Tissue Engineering for Hair Follicles Regeneration

November 2022 in “Regenerative Therapy”

Advancements in tissue engineering show promise for hair follicle regeneration to treat hair loss.

research Hair Follicle Stem Cell Cultures Reveal Self-Organizing Plasticity of Stem Cells and Their Progeny

61 citations, December 2016 in “The EMBO Journal”

The study showed that hair follicle stem cells can maintain and organize themselves in a lab setting, keeping their ability to renew and form hair and skin.

research New Substrates for Stem Cell Control

11 citations, May 2018 in “Philosophical Transactions of the Royal Society B”

New materials help control stem cell growth and specialization for medical applications.

research Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells

2 citations, January 2020 in “Methods in molecular biology”

Scientists created early-stage hair follicles from human skin cells, which could help treat baldness and study hair growth.

research The Role of 3Rs in the Age of One Health: Where We Are and Where We’re Going

October 2023 in “Biomedical science and engineering”

Innovative methods are reducing animal testing and improving biomedical research.

Clonal Growth of Dermal Papilla Cells in Hydrogels Reveals Intrinsic Differences Between Sox2-Positive and Sox2-Negative Cells In Vitro and In Vivo

research Clonal Growth of Dermal Papilla Cells in Hydrogels Reveals Intrinsic Differences Between Sox2-Positive and Sox2-Negative Cells In Vitro and In Vivo

68 citations, December 2011 in “Journal of Investigative Dermatology”

Sox2-positive dermal papilla cells have unique characteristics and contribute more to skin and hair follicle formation than Sox2-negative cells.

research Dermal Papilla Cells and Melanocytes Response to Physiological Oxygen Levels Depends on Their Interactions

7 citations, June 2021 in “Cell Proliferation”

Low oxygen levels improve the function of hair and skin cells when they are in direct contact.

research Thyrotropin-Releasing Hormone Promotes Wound Re-Epithelialisation in Frog and Human Skin

46 citations, September 2013 in “PLOS ONE”

Thyrotropin-Releasing Hormone helps heal wounds in frog and human skin.

research Large-Scale Cultivation of Transplantable Dermal Papilla Cellular Aggregates Using Microfabricated PDMS Arrays

25 citations, August 2010 in “Acta Biomaterialia”

Researchers developed a method to grow hair follicle cells for transplantation using a special chip.

research Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro

1 citations, January 2018 in “Methods in molecular biology”

The research found ways to activate melanocyte stem cells for potential treatment of skin depigmentation conditions.

research Enhanced Expansion and Sustained Inductive Function of Skin-Derived Precursor Cells in Computer-Controlled Stirred Suspension Bioreactors

17 citations, September 2016 in “Stem cells translational medicine”

Using bioreactors, scientists can grow more skin stem cells that keep their ability to regenerate skin and hair.

research Controllable Production of Transplantable Adult Human High-Passage Dermal Papilla Spheroids Using 3D Matrigel Culture

46 citations, September 2014 in “Tissue engineering. Part A”

Researchers created hair-inducing human cell clusters using a 3D culture method.

research Hair Follicle Culture: A Historical Perspective

1 citations, July 2013 in “Hair transplant forum international”

Advancements in hair follicle culture have led to better understanding and potential treatments for hair loss.

research Hair Follicle Culture

January 2012 in “Methods in pharmacology and toxicology”

Hair follicle culture helps study hair growth but has limitations in modeling the full hair cycle.

research Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

18 citations, November 2013 in “Molecules and Cells”

New culture method keeps human skin stem cells more stem-like.

research Impact of Adipose-Derived Stem Cells on Engineering Hair Follicle Germ-Like Tissue Grafts for Hair Regenerative Medicine

11 citations, March 2021 in “Journal of Bioscience and Bioengineering”

Adding human fat-derived stem cells to hair follicle grafts greatly increases hair growth.