Establishment of an Efficient Primary Culture System for Human Hair Follicle Stem Cells Using the Rho-Associated Protein Kinase Inhibitor Y-27632

    Lihong Wen, Yong Miao, Zhexiang Fan … Ruosi Chen
    Studysummary This study established an efficient short-term culture system for human hair follicle stem cells using human fibronectin and ROCK inhibitor Y-27632, enhancing cell proliferation and stemness for potential applications in hair follicle tissue engineering.
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    Research cited in this study 20

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    4. Tissue Engineering of Human Hair Follicles Using a Biomimetic Developmental Approach Nature Communications · 2018
    5. Hedgehog Stimulates Hair Follicle Neogenesis by Creating Inductive Dermis During Murine Skin Wound Healing Nature Communications · 2018
    6. Therapeutic Potential of Stem Cells in Follicle Regeneration Stem Cells International · 2018
    7. ROCK Inhibitor Y-27632 Maintains the Propagation and Characteristics of Hair Follicle Stem Cells PubMed · 2018
    8. Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices Cell · 2017
    9. Hair Follicle Stem Cell Cultures Reveal Self-Organizing Plasticity of Stem Cells and Their Progeny The EMBO Journal · 2016
    10. Neonatal Murine Skin-Derived Cells Transplanted Using a Mini-Chamber Model Produce Robust and Normal Hair Journal of Tissue Engineering and Regenerative Medicine · 2013
    11. Hair Follicle: A Novel Source of Multipotent Stem Cells for Tissue Engineering and Regenerative Medicine Tissue Engineering Part B-reviews · 2012
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    14. Bald Scalp in Men with Androgenetic Alopecia Retains Hair Follicle Stem Cells but Lacks CD200-Rich and CD34-Positive Hair Follicle Progenitor Cells Journal of Clinical Investigation · 2011
    15. Morphometric Analysis of the Human Scalp Hair Follicle: Practical Implications for the Hair Transplant Surgeon and Hair Regeneration Studies Dermatologic Surgery · 2010
    16. Defining the Hair Follicle Stem Cell (Part I) Journal of Cutaneous Pathology · 2009
    17. Isolation of a Novel Population of Multipotent Adult Stem Cells from Human Hair Follicles American Journal Of Pathology · 2006
    18. Characterization and Isolation of Stem Cell-Enriched Human Hair Follicle Bulge Cells ˜The œJournal of clinical investigation/˜The œjournal of clinical investigation · 2005
    19. In Vitro Differences Between Keratinocyte Stem Cells and Transit-Amplifying Cells of the Human Hair Follicle Journal of Investigative Dermatology · 2005
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