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    Glossary 3D cell culture

    technique growing cells in three dimensions for realistic conditions

    3D cell culture is a technique used to grow cells in an environment that more closely mimics the natural conditions inside the human body compared to traditional two-dimensional (2D) cultures. By allowing cells to interact in all three dimensions, this method provides a more accurate representation of how cells behave, grow, and respond to treatments, making it particularly valuable for research in areas like cancer, tissue engineering, and drug development.

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    1. 3D co-culture model of endothelial colony-forming cells (ECFCs) reverses late passage adipose-derived stem cell senescence for wound healing Stem Cell Research & Therapy · 2020 · 17 citations
    2. 3D cell culture stimulates the secretion of in vivo like extracellular vesicles bioRxiv (Cold Spring Harbor Laboratory) · 2019 · 5 citations
    3. Comparative analysis of 3D and 2D cell-culturing methods in hair follicle spheroid morphogenesis and drug responsiveness Biomaterials Advances · 2025 · 1 citations
    4. 3D Co-Culture model of Endothelial Colony Forming Cells (ECFCs) Reverses Late Passage Adipose-derived Stem Cell Senescence for Wound Healing Research Square (Research Square) · 2020
    5. In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture models Frontiers in Bioengineering and Biotechnology · 2023
    6. Bioinspired tuning of glycol chitosan for 3D cell culture NPG Asia Materials · 2016 · 61 citations
    7. Mitochondrial Oxygen Consumption and Immunocytochemistry of Human Dental Pulp Stem Cell Following 808 nm PBM Therapy: A 3D Cell Culture Study Journal of Biophotonics · 2025
    8. Keratinocytes maintain compartmentalization between dermal papilla and fibroblasts in 3D heterotypic tri‐cultures Cell Proliferation · 2019 · 16 citations
    9. 3D Spheroid Human Dermal Papilla Cell as an Effective Model for the Screening of Hair Growth Promoting Compounds: Examples of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid (TCQA) Cells · 2022 · 2 citations
    10. Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering Nanomaterials · 2024 · 1 citations
    11. Peer Review #1 of "Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy (v0.1)" 2018
    12. Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles Experimental Dermatology · 2017 · 55 citations
    13. In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers Scientific Reports · 2017 · 14 citations
    14. Hair follicular cell/organ culture in tissue engineering and regenerative medicine Biochemical Engineering Journal · 2009 · 21 citations
    15. CellTissueScience 2018: Innovations in regenerative medicine that improve the results of stem cell treatment: 3D cultivation, in vivo modeling of stem cell niche and prenatal stem cells exosomes_AbhijitBopardikar_ReeLabs Pvt. Ltd., India Cell & developmental biology · 2019
    16. 1333 Spheroids cell cultures as a promising study model for hair follice regeneration Journal of Investigative Dermatology · 2018
    17. Extracellular vesicles from 3D cultured dermal papilla cells improve wound healing via Krüppel-like factor 4/vascular endothelial growth factor A -driven angiogenesis Burns and trauma · 2023 · 1 citations
    18. Genome-wide expression profile analysis of 3D cultured dermal papilla cells from patients with androgenic alopecia Chinese Journal of Dermatology · 2014
    19. Hair follicle stem cell cultures reveal self‐organizing plasticity of stem cells and their progeny The EMBO Journal · 2016 · 61 citations
    20. 195 Androgen action and 3D culture conditions influence dermal papilla cells inductive properties: the role of Wnt agonists/antagonists balance Journal of Investigative Dermatology · 2016
    21. Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth Frontiers in Cell and Developmental Biology · 2021 · 5 citations
    22. Programmable viscoelastic hydrogels exhibit antimicrobial and regenerative properties to promote cell migration, wound healing, and tissue remodeling Microsystems & Nanoengineering · 2026
    23. Evaluating the Characteristics of Human Hair Follicle Dermal Papilla Cells in Aggregation Culture Procedure Biomedical and Biotechnology Research Journal (BBRJ) · 2026
    24. “Two-Cell Assemblage” Assay: A Simple in vitro Method for Screening Hair Growth-Promoting Compounds Frontiers in Cell and Developmental Biology · 2020 · 5 citations
    25. Novel in vitro culture condition improves the stemness of human dermal stem/progenitor cells Molecules and Cells · 2013 · 18 citations
    26. Isolation of epithelial stem cells from dermis by a three-dimensional culture system Journal of Cellular Biochemistry · 2006 · 38 citations
    27. Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair BMC Biotechnology · 2024 · 5 citations
    28. WNT10A promotes an invasive and self-renewing phenotype in esophageal squamous cell carcinoma Carcinogenesis · 2015 · 55 citations
    29. Wnt1a maintains characteristics of dermal papilla cells that induce mouse hair regeneration in a 3D preculture system Journal of Tissue Engineering and Regenerative Medicine · 2015 · 23 citations
    30. Mesenchymal stem cells paracrine proteins from three‐dimensional dynamic culture system promoted wound healing in third‐degree burn models Bioengineering & translational medicine · 2023