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Glossary3D cell culture (Spheroid Culture)

technique growing cells in three dimensions for realistic conditions

3D cell culture is a technique used to grow cells in an environment that more closely mimics the natural conditions inside the human body compared to traditional two-dimensional (2D) cultures. By allowing cells to interact in all three dimensions, this method provides a more accurate representation of how cells behave, grow, and respond to treatments, making it particularly valuable for research in areas like cancer, tissue engineering, and drug development.

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Enhanced Viability and Neural Differentiation Potential in Poor Post-Thaw Human Adipose-Derived Stem Cells by Agarose Multi-Well Dishes and Spheroid Culture

research Enhanced Viability and Neural Differentiation Potential in Poor Post-Thaw Human Adipose-Derived Stem Cells by Agarose Multi-Well Dishes and Spheroid Culture

15 citations , June 2015 in “Human Cell”

Spheroid culture in agarose dishes improves survival and nerve cell growth in thawed human fat-derived stem cells.

Titrated Extract of Centella Asiatica Increases Hair Inductive Property Through Inhibition of STAT Signaling Pathway in Three-Dimensional Spheroid Cultured Human Dermal Papilla Cells

research Titrated Extract of Centella Asiatica Increases Hair Inductive Property Through Inhibition of STAT Signaling Pathway in Three-Dimensional Spheroid Cultured Human Dermal Papilla Cells

13 citations , October 2017 in “Bioscience, Biotechnology, and Biochemistry”

Centella asiatica extract may help promote hair growth by blocking a specific cell signaling pathway.

research Flavonoid Silibinin Increases Hair-Inductive Property Via Akt and Wnt/β-Catenin Signaling Activation in 3-Dimensional Spheroid Cultured Human Dermal Papilla Cells

29 citations , February 2019 in “Journal of Microbiology and Biotechnology”

Silibinin may help hair growth and treat hair loss.

In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers

research In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers

14 citations , April 2017 in “Scientific Reports”

Using a perfusion system and 3D spheroid culture improves the growth of corneal cell layers for tissue engineering.

research 3D Spheroid Human Dermal Papilla Cell as an Effective Model for the Screening of Hair Growth Promoting Compounds: Examples of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid (TCQA)

2 citations , June 2022 in “Cells”

3D cell cultures are better for testing hair growth treatments than 2D cultures.

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community Surprisingly thorough study on He-Shou-Wu/Fo Ti

in Research/Science 14 upvotes 2 years ago

He Shou Wu (Fo Ti) extract was found to prolong the hair growth phase, inhibit 5-alpha-reductase (like finasteride), reduce androgen receptors, and increase growth factors, potentially outperforming minoxidil in recovering hair follicle size after DHT exposure. Two compounds, emodin and TSG, are identified as responsible for these effects and warrant further investigation.

community Why the cure is not yet found and why it is always 5 years away? Why no hopes on PP405, GT, KX, Hair cloning, AMP, etc, etc, etc

in Chat 19 upvotes 10 months ago

Skepticism about hair loss research motivations, suggesting financial interests hinder finding a cure. Mentions treatments like minoxidil, finasteride, and dutasteride, but notes they are not cures.