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Glossary3D cell culture (Spheroid Culture)

technique growing cells in three dimensions for realistic conditions

3D cell culture is a technique used to grow cells in an environment that more closely mimics the natural conditions inside the human body compared to traditional two-dimensional (2D) cultures. By allowing cells to interact in all three dimensions, this method provides a more accurate representation of how cells behave, grow, and respond to treatments, making it particularly valuable for research in areas like cancer, tissue engineering, and drug development.

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Enhanced Viability and Neural Differentiation Potential in Poor Post-Thaw Human Adipose-Derived Stem Cells by Agarose Multi-Well Dishes and Spheroid Culture

research Enhanced Viability and Neural Differentiation Potential in Poor Post-Thaw Human Adipose-Derived Stem Cells by Agarose Multi-Well Dishes and Spheroid Culture

15 citations, June 2015 in “Human Cell”

Spheroid culture in agarose dishes improves survival and nerve cell growth in thawed human fat-derived stem cells.

Titrated Extract of Centella Asiatica Increases Hair Inductive Property Through Inhibition of STAT Signaling Pathway in Three-Dimensional Spheroid Cultured Human Dermal Papilla Cells

research Titrated Extract of Centella Asiatica Increases Hair Inductive Property Through Inhibition of STAT Signaling Pathway in Three-Dimensional Spheroid Cultured Human Dermal Papilla Cells

13 citations, October 2017 in “Bioscience, Biotechnology, and Biochemistry”

Centella asiatica extract may help promote hair growth by blocking a specific cell signaling pathway.

research 3D Spheroid Human Dermal Papilla Cell as an Effective Model for the Screening of Hair Growth Promoting Compounds: Examples of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid (TCQA)

2 citations, June 2022 in “Cells”

3D cell cultures are better for testing hair growth treatments than 2D cultures.

Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid

research Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid

November 2021 in “Research Square (Research Square)”

3D spheroid cultures of human hair follicle cells are better for hair growth research than 2D cultures, and they provide new insights into how hair growth treatments like minoxidil and TCQA work.

research TGF-β2 and Collagen Play Pivotal Roles in the Spheroid Formation and Anti-Aging of Human Dermal Papilla Cells

11 citations, August 2021 in “Aging”

Collagen and TGF-β2 help maintain hair cell shape and youthfulness.

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community Surprisingly thorough study on He-Shou-Wu/Fo Ti

in Research/Science 14 upvotes 1 year ago

He Shou Wu (Fo Ti) extract was found to prolong the hair growth phase, inhibit 5-alpha-reductase (like finasteride), reduce androgen receptors, and increase growth factors, potentially outperforming minoxidil in recovering hair follicle size after DHT exposure. Two compounds, emodin and TSG, are identified as responsible for these effects and warrant further investigation.