Enhanced Viability and Neural Differentiation Potential in Poor Post-Thaw Human Adipose-Derived Stem Cells by Agarose Multi-Well Dishes and Spheroid Culture

June 2015 in “ Human Cell ”

Xinhong Guo, Shanyi Li, Qingshan Ji, Ruiling Lian, Jiansu Chen

TLDR Spheroid culture in agarose dishes improves survival and nerve cell growth in thawed human fat-derived stem cells.

The study from 2015 investigated the effects of spheroid culture on the viability and neural differentiation potential of human adipose-derived stem cells (hADSCs) after cryopreservation. The researchers used agarose multi-well dishes to form hADSC spheroids and found that these spheroids exhibited increased cell proliferation, viability, and anti-apoptosis compared to non-spheroid hADSCs. Additionally, the spheroid-cultured hADSCs showed enhanced neural differentiation potential after 3 days of induction, without significant changes in their typical stem cell markers or adipogenic and osteogenic differentiation potential. The study concluded that spheroid culture using agarose multi-well dishes is a simple and cost-effective method to improve the post-thaw performance of hADSCs, which could be beneficial for cell-based therapies. The number of cells or participants used in the study was not mentioned in the summary.

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