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    Glossary 2D cell culture

    cells grown on flat surfaces for research purposes

    2D cell culture, also known as two-dimensional cell culture, is a laboratory technique where cells are grown on a flat, typically plastic, surface such as a petri dish. This method allows researchers to study cell behavior, drug responses, and biological processes in a controlled environment, but it may not fully replicate the complex interactions found in a living organism.

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    1. Comparative analysis of 3D and 2D cell-culturing methods in hair follicle spheroid morphogenesis and drug responsiveness Biomaterials Advances · 2025 · 1 citations
    2. Peer Review #1 of "Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy (v0.1)" 2018
    3. Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth Frontiers in Cell and Developmental Biology · 2021 · 5 citations
    4. Novel in vitro culture condition improves the stemness of human dermal stem/progenitor cells Molecules and Cells · 2013 · 18 citations
    5. Culture Strategy Determines the Differentiation Status of Sweat Gland Cells Cells · 2025
    6. 3D cell culture stimulates the secretion of in vivo like extracellular vesicles bioRxiv (Cold Spring Harbor Laboratory) · 2019 · 5 citations
    7. Three-dimensional models: from cell culture to Patient-Derived Organoid and its application to future liposarcoma research Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics · 2024 · 4 citations
    8. Human hair keratin hydrogel : fabrication, characterization and application 2014
    9. Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods Skin Pharmacology and Physiology · 2020 · 35 citations
    10. Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth Proceedings of the National Academy of Sciences of the United States of America · 2013 · 256 citations
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