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    Glossary 2D cell culture

    cells grown on flat surfaces for research purposes

    2D cell culture, also known as two-dimensional cell culture, is a laboratory technique where cells are grown on a flat, typically plastic, surface such as a petri dish. This method allows researchers to study cell behavior, drug responses, and biological processes in a controlled environment, but it may not fully replicate the complex interactions found in a living organism.

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    1. Comparative analysis of 3D and 2D cell-culturing methods in hair follicle spheroid morphogenesis and drug responsiveness Biomaterials Advances · 2025 · 1 citations
    2. Peer Review #1 of "Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy (v0.1)" 2018
    3. Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth Frontiers in Cell and Developmental Biology · 2021 · 5 citations
    4. Novel in vitro culture condition improves the stemness of human dermal stem/progenitor cells Molecules and Cells · 2013 · 18 citations
    5. Culture Strategy Determines the Differentiation Status of Sweat Gland Cells Cells · 2025
    6. 3D cell culture stimulates the secretion of in vivo like extracellular vesicles bioRxiv (Cold Spring Harbor Laboratory) · 2019 · 5 citations
    7. Three-dimensional models: from cell culture to Patient-Derived Organoid and its application to future liposarcoma research Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics · 2024 · 4 citations
    8. Human hair keratin hydrogel : fabrication, characterization and application 2014
    9. Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods Skin Pharmacology and Physiology · 2020 · 35 citations
    10. Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth Proceedings of the National Academy of Sciences of the United States of America · 2013 · 256 citations
    11. 3D Spheroid Human Dermal Papilla Cell as an Effective Model for the Screening of Hair Growth Promoting Compounds: Examples of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid (TCQA) Cells · 2022 · 2 citations
    12. Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering Nanomaterials · 2024 · 1 citations
    13. Evaluating the Characteristics of Human Hair Follicle Dermal Papilla Cells in Aggregation Culture Procedure Biomedical and Biotechnology Research Journal (BBRJ) · 2026
    14. Detecting the Mechanism behind the Transition from Fixed Two-Dimensional Patterned Sika Deer (Cervus nippon) Dermal Papilla Cells to Three-Dimensional Pattern International Journal of Molecular Sciences · 2021 · 2 citations
    15. CellTissueScience 2018: Innovations in regenerative medicine that improve the results of stem cell treatment: 3D cultivation, in vivo modeling of stem cell niche and prenatal stem cells exosomes_AbhijitBopardikar_ReeLabs Pvt. Ltd., India Cell & developmental biology · 2019
    16. Faculty Opinions recommendation of Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · 2013
    17. Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-caffeoylquinic acid (TCQA) Research Square (Research Square) · 2021
    18. Fish Cell Spheroids, a Promising In Vitro Model to Mimic In Vivo Research: A Review Cells · 2024 · 5 citations
    19. In pemphigus, cell detachment, but not autoantibody binding, induces cell-wide, long-lasting transcriptomic and proteomic changes bioRxiv (Cold Spring Harbor Laboratory) · 2025
    20. Three-Dimensional-Bioprinted Non-Small Cell Lung Cancer Models in a Mouse Phantom for Radiotherapy Research International Journal of Molecular Sciences · 2024 · 5 citations
    21. Nanoscale microenvironment engineering for expanding human hair follicle stem cell and revealing their plasticity Journal of Nanobiotechnology · 2021 · 15 citations
    22. 3D spheroid culture synchronizes heterogeneous MSCs into an immunomodulatory phenotype with enhanced anti-inflammatory effects iScience · 2024 · 13 citations
    23. Analysis of Dermal Papilla Cell Interactome Using STRING Database to Profile the ex Vivo Hair Growth Inhibition Effect of a Vinca Alkaloid Drug, Colchicine International Journal of Molecular Sciences · 2015 · 23 citations
    24. Profiling the Response of Human Hair Follicles to Ultraviolet Radiation Journal of Investigative Dermatology · 2009 · 53 citations
    25. Microfluidic Technology for the Generation of Cell Spheroids and Their Applications Micromachines · 2017 · 125 citations
    26. Cell observation method under near-living conditions by scanning electron microscopy Microscopy · 2014
    27. Application and Progress of Cultured Models of Gallbladder Carcinoma Journal of Clinical and Translational Hepatology · 2023 · 8 citations
    28. Spherical Skin Model: Stratified Co‐Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening Advanced Healthcare Materials · 2025
    29. Improvement of Antioxidant Defences in Keratinocytes Grown in Physioxia: Comparison of 2D and 3D Models Oxidative Medicine and Cellular Longevity · 2023 · 9 citations
    30. Establishment of an in vitro organoid model of dermal papilla of human hair follicle Journal of Cellular Physiology · 2018 · 44 citations