TLDR 3D microenvironments in microwells improve hair follicle stem cell behavior and hair regeneration.
This study investigates the effects of a three-dimensional (3D) microenvironment on hair follicle (HF) regeneration by culturing HF-derived cells, primarily hair follicle stem cells (HFSCs), in 3D spheroids using a microwell array. The research focuses on the expression of Sonic hedgehog signaling and stem cell markers in these spheroids, as well as the impact of microwell depth on spheroid formation and biological conditions. Computational fluid dynamics were used to predict biomolecular diffusion and convective flow, providing insights into the physical stimulations at the micro-scale. The findings offer fundamental insights into HF physiology in a 3D environment and highlight the importance of geometric parameters in microwell systems for HF research.
1 citations,
February 2023 in “ACS Biomaterials Science & Engineering” The new microwell device helps grow more hair stem cells that can regenerate hair.
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