Extracellular Vesicles From 3D Cultured Dermal Papilla Cells Improve Wound Healing Via Krüppel-Like Factor 4/Vascular Endothelial Growth Factor A-Driven Angiogenesis

    January 2023 in “ Burns and trauma ”
    Yunwei Wang, Kuo Shen, Yi Sun, Peng Cao, Zhiyuan Jia, Wanfu Zhang, Yang Liu, Hao Zhang, Chen Yang, Shaohui Li, Chaolei Xu, Chunmao Han, Yating Qiao, Qingyi Zhang, Boyu Wang, Liang Luo, Yunshu Yang, Hao Guan
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    TLDR Tiny particles from 3D-grown skin cells speed up wound healing by promoting blood vessel growth.
    This study explored the use of extracellular vesicles (EVs) from 3D cultured dermal papilla cells (tdDPCs) to improve wound healing. The researchers developed tdDPCs using a self-feeder 3D culture method and extracted EVs from these cells. They compared the effects of EVs from tdDPCs and traditional DPCs on human umbilical vein endothelial cells (HUVECs) and in a murine model of full-thickness skin injury. The tdDPC-EVs were found to significantly promote HUVEC proliferation, lumen formation, and migration, and they enhanced angiogenesis and wound healing more effectively than DPC-EVs both in vitro and in vivo. Bioinformatics analysis and functional experiments indicated that the tdDPC-EV-regulated Krüppel-like factor 4 (KLF4)/vascular endothelial growth factor A (VEGFA) axis is crucial for accelerating wound healing. The study concludes that 3D cultivation is a promising strategy for developing DPC-derived EVs for skin wound treatment, with tdDPC-EVs notably improving wound healing through KLF4/VEGFA-driven angiogenesis.
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