Rescuing Key Native Traits in Cultured Dermal Papilla Cells for Human Hair Regeneration

    May 2021 in “ Journal of Advanced Research
    Carla M. Abreu, Mariana T. Cerqueira, Rogério P. Pirraco, Luca Gasperini, Rui L. Reis, Alexandra P. Marques
    TLDR Conditioned medium from keratinocytes can improve hair growth potential in cultured dermal papilla cells.
    The study investigated the potential of conditioned medium from interfollicular keratinocytes (KCs-CM) to restore the hair inductive properties of cultured human dermal papilla (DP) cells, which typically lose this ability in an epithelium-deficient environment. DP cells cultured with KCs-CM showed improved hair-inductive capacity, higher alkaline phosphatase activity, better self-aggregation, and a secretome profile similar to that during the anagen phase. These cells also supported the formation of hair follicle and sebaceous gland-like structures when grafted with human keratinocytes in nude mice. The findings suggested that KCs-CM could enhance DP cell function more effectively than conventional 3D cultures, offering a promising approach for hair follicle regeneration and wound management.
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