Extracellular Vesicles from Hair Follicle-Derived Mesenchymal Stromal Cells: Isolation, Characterization, and Therapeutic Potential for Chronic Wound Healing
April 2022
in “
Stem cell research & therapy
”
extracellular vesicles hair follicle mesenchymal stromal cells chronic wound healing medium-large EVs small EVs adipose tissue-derived MSCs human dermal fibroblasts angiogenesis human umbilical vein endothelial cells oxidative stress cytotoxicity hyperglycemic environment EVs HF-MSCs chronic wounds m-lEVs sEVs AT-MSCs HDFs HUVECs
TLDR Hair follicle-derived extracellular vesicles may help heal chronic wounds as effectively as those from adipose tissue.
The study investigates the therapeutic potential of extracellular vesicles (EVs) derived from hair follicle mesenchymal stromal cells (HF-MSCs) for chronic wound healing. Researchers isolated and characterized medium-large EVs (m-lEVs) and small EVs (sEVs) from HF-MSCs and compared them to EVs from adipose tissue-derived MSCs (AT-MSCs). Both HF-EVs and AT-EVs expressed typical MSC-EV markers and increased human dermal fibroblasts (HDFs) proliferation and migration, enhanced angiogenesis in human umbilical vein endothelial cells (HUVECs), and protected HDFs from oxidative stress and cytotoxicity in a hyperglycemic environment. The findings suggest that HF-EVs have comparable regenerative potential to AT-EVs, making them promising candidates for chronic wound treatment.
