Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation
January 2022
in “
Biomedicines
”
human hair follicle-derived mesenchymal stromal cells HF-MSCs lower dermal sheath immunomodulation neural marker expression mesenchymal stem cell criteria chondrogenic differentiation osteogenic differentiation adipogenic differentiation pro-inflammatory stimuli IDO MHC-II expression immunoevasiveness PBMC proliferation cytotoxic T cells B cells regulatory T cells adipose tissue-derived MSCs AT-MSCs M2 macrophage polarization hair follicle-derived MSCs stem cell criteria
TLDR Cells from the lower part of hair follicles are a promising, less invasive option for immune system therapies.
The study investigates the potential of human hair follicle-derived mesenchymal stromal cells (HF-MSCs) from the lower dermal sheath for immunomodulation. HF-MSCs were isolated and characterized, showing neural marker expression and fulfilling mesenchymal stem cell criteria. They demonstrated the ability to differentiate into chondrogenic, osteogenic, and adipogenic lineages, although stemness properties decreased over time in culture. HF-MSCs responded to pro-inflammatory stimuli by upregulating IDO and maintaining low MHC-II expression, indicating immunoevasiveness. They inhibited PBMC proliferation, reduced cytotoxic T cells and B cells, and induced regulatory T cells more effectively than adipose tissue-derived MSCs (AT-MSCs). HF-MSCs also promoted M2 macrophage polarization better than AT-MSCs, suggesting their superior immunomodulatory potential.
