Three-Dimensional Cultured Human Dermal Papilla Cells in HGC-Coated Environments Enhance Hair Regeneration and Artificial Skin Integration

January 2024 in “ Biomaterials Research ”

Thuy Trang Truong, Hee-Jin Park, Kyoung Hwan Park, Jin Jung Song, Byoung‐Seok Lee, Kang Moo Huh, Sun‐Woong Kang

hexanoyl glycol chitosan HGC human hair follicle dermal papilla cells HDP cells 3D spheroids hair-like structures minoxidil keratinocytes artificial skin functional papilla structures dermal papilla cells Rogaine

TLDR 3D-cultured cells in HGC-coated environments improve hair growth and skin integration.

The study utilized hexanoyl glycol chitosan (HGC)-coated dishes to cultivate human hair follicle dermal papilla (HDP) cells in 3D spheroids, which successfully formed hair-like structures. Minoxidil treatment further enhanced hair growth in these spheroids when cocultured with keratinocytes. Additionally, transplantation of the spheroids into artificial skin resulted in the formation of functional papilla structures, indicating a closer mimicry of human skin. These findings suggest promising advancements in hair loss treatment, cosmetic and pharmaceutical evaluations, and skin restoration therapies.

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