Detecting the Mechanism Behind the Transition from Fixed Two-Dimensional Patterned Sika Deer (Cervus Nippon) Dermal Papilla Cells to Three-Dimensional Pattern

The study explored the transition of sika deer dermal papilla cells from a two-dimensional (2D) to a three-dimensional (3D) pattern, which is crucial for hair follicle development. It was found that the culture conditions can influence the growth pattern of these cells, with the ability to revert to a 3D pattern and regain hair-inducing properties when conditions are altered. Transcriptome analysis revealed 576 differentially expressed genes between aggregated and unaggregated cells, with up-regulated genes involved in cell adhesion, extracellular matrix organization, and signaling pathways. The study confirmed that the self-aggregative behavior of DP cells is not species-specific and can be induced by changing the culture environment. This has potential implications for human hair follicle neogenesis. The research was conducted on primary DP cell lines established from skin tissues of 15 farmed male sika deer, aged 2-3 years, and used cells between passage two and eight.