Primary Cell Culture and Morphological Characterization of Canine Dermal Papilla Cells and Dermal Fibroblasts

    February 2002 in “ Veterinary Dermatology ”
    Christine B. Bratka‐Robia, Gerda Mitteregger, Amanda Aichinger, Monika Egerbacher, M. Helmreich, E. Bamberg
    TLDR Canine dermal papilla cells and fibroblasts have distinct growth patterns and protein expressions.
    The study focused on the primary cell culture and morphological characterization of canine dermal papilla cells (DPC) and dermal fibroblasts (DFB) from female dogs. DPCs were isolated from primary hair follicles and cultured in Amniomax complete C100 medium, reaching confluence in 3 weeks and subcultivated every 7 days. DFBs were cultured from interfollicular dermis in serum-free medium, reaching confluence in 10 days and subcultivated every 5 days. Immunohistochemistry revealed that DPCs exhibited an aggregative growth pattern with pseudopapillae formation and strong staining for collagen IV and laminin up to the sixth passage, while DFBs grew in branching, parallel lines with weak staining for these proteins.
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