Integrating Stem Cell Biology and Organoid Technology for Skin Regenerative Therapies

This study found that generating pluripotent stem cell-derived skin organoids in a 3D bioprinted hydrogel device improved keratinocyte differentiation and hair follicle formation while reducing necrosis.

In this study, iPSC-derived epidermal organoids demonstrated potential as a novel tool for regenerative medicine by producing high-performance extracellular vesicles that promote proliferation, migration, and angiogenesis in cell-free therapies for wound healing.

In this study, researchers developed a microfluidic-assisted technology to create dual-layer microspheres containing mesenchymal and epidermal cells, which efficiently induced hair follicle regeneration in mice, suggesting potential improvements for hair regeneration treatments in conditions like androgenetic alopecia.

This study observed that in aged mouse skin, there was an increase in IL-17-expressing T helper cells, γδ T cells, and innate lymphoid cells, and blocking IL-17 signaling reduced skin inflammation and delayed age-related changes, suggesting it as a potential target to mitigate skin aging.

This study found that organoid cultures of human epithelial and mesenchymal cells can produce hair peg-like structures, and their growth is enhanced by minoxidil, suggesting potential for hair drug screening.

This review discusses the role of collagen XVII in maintaining stem cell niches and its impact on skin aging and wound repair, without reporting new clinical results.

This review discusses the current state of research on skin organoids, highlighting their potential for replicating complex skin structures and comparing recent studies on stem cell microenvironments.

Scientists created human skin with hair from stem cells, which could help treat hair loss and skin conditions.

This study found that their system effectively supports the rapid formation of human hair follicle organoids, providing a platform for studying hair regeneration and drug screening.

This study introduces a method for long-term expansion of adult mouse epidermal stem cells in vitro using an organoid culture system that maintains the basal-apical organization.

This study suggests that in hidradenitis suppurativa, MMP-induced matrix alterations may contribute to inflammation by releasing active peptides and inflammatory factors in the extracellular matrix.

In this study, researchers developed a new 3D skin equivalent assay using mouse epidermal and dermal cells that successfully regenerated organized hair follicles, outperforming a 2D model in hair follicle orientation and quantity, and enhanced hair growth when Wnt3a was added.

This study developed an in vitro system to analyze human skin progenitor cells' ability to form hair peg-like structures, providing insights for engineering hair follicle organoids.

This study demonstrated that enhancing human skin constructs with hair follicles through engineered cell organization and vascularization improved hair growth in immunodeficient mice, suggesting potential advances for treating alopecia and chronic wounds.

In this study, researchers successfully derived skin organoids from mouse pluripotent stem cells that spontaneously produce hair follicles, potentially aiding in hair growth research and in vitro drug testing.

This study demonstrated a method for large-scale preparation of self-sorted hair follicle germs in vitro, which successfully generated hair follicles when transplanted into the skin of nude mice.

This study found that newborn mouse skin organoids can robustly grow hair in vivo, while adult organoids require specific interventions to restore their hair-forming ability.

This study shows that macrophage-induced TNF signaling can activate hair follicle stem cells and promote new hair follicle formation after wounding by regulating AKT/β-catenin pathways.

In this study, researchers differentiated induced pluripotent stem cells into cells with dermal papilla-like properties, demonstrating their potential role in hair follicle bioengineering and drug testing for hair growth.

In this study, the researchers found that using a core-shell assembly of adipose-derived stem cells and dermal papilla cells reconstructed hair follicle cellular arrangements more effectively than mixed assemblies, enhancing hair formation in vivo.

Researchers created a fully functional, bioengineered skin system with hair from stem cells that successfully integrated when transplanted into mice.

This research found that Foxc1 transcription factor and COL17A1 are critical in regulating quiescence and hair thinning in hair follicle stem cells, with aging-related DNA damage leading to hair loss.

This essay discusses the establishment, maintenance, and role of skin stem cells in tissue homeostasis, wound repair, and their relation to skin cancer, without reporting new experimental findings.

This study found that super-enhancers are essential for hair follicle stem cell identity, lineage commitment, and plasticity in mice, with SOX9 being a key regulator of these chromatin dynamics.

This narrative review examines the interactions between skin stem cells and their niches, finding new insights from advancements in genetic and imaging tools but reporting no new experimental results.

This study found that aging in the epidermis disrupts cytokine balance and stem cell function, which may contribute to broader tumor-suppressive mechanisms.

This study demonstrated that bioengineered hair follicles reconstituted from embryonic skin cells and transplanted into hosts can restore physiological hair functions, suggesting potential applications for treating alopecia.

COL17A1 is crucial for preventing hair graying and loss by supporting hair and pigment stem cells.

This study suggests that Lrig1-positive cells form a previously unknown reservoir of interfollicular epidermal stem cells in adult mouse skin, influencing epidermal proliferation and hair follicle development.

Lgr5 is a marker for active, long-lasting stem cells in mouse hair follicles.

Hair follicles can regrow in wounded adult mouse skin using a process like embryo development.