Biomimetic Biphasic Microsphere Preparation for Hair Regeneration

The study developed a co-flowing microfluidic-assisted technology to produce dual aqueous microdroplets for hair regeneration. These microspheres, named G/HAD, were created using glycosaminoglycan-based photo-crosslinkable biological macromolecule (HAD) shells and gelatin methacrylate (GelMA) cores to enclose mesenchymal cells (MSCs) and mouse epidermal cells (EPCs). The G/HAD microspheres showed favorable characteristics, including sustained growth factor release and wet adhesion properties. After being transplanted into the dorsal skin of BALB/c nude mice, the G/HAD cell microspheres efficiently induced the regeneration of hair follicles. This method allows for the production of approximately 250 dual-layer microspheres per minute, showing potential in improving current hair regeneration techniques.