Biomimetic Biphasic Microsphere Preparation for Hair Regeneration

February 2024 in “ International Journal of Biological Macromolecules ”

Yangpeng Chen, Danlan Fu, Xiaoqi Wu, Yufan Zhang, Yuxin Chen, Yi Zhou, Mengji Lu, Qifa Liu, Junfei Huang

glycosaminoglycan gelatin methacrylate mesenchymal cells mouse epidermal cells growth factor release hair follicles hair regeneration G/HAD microspheres GelMA MSCs EPCs

Studysummary In this study, researchers developed a microfluidic-assisted technology to create dual-layer microspheres containing mesenchymal and epidermal cells, which efficiently induced hair follicle regeneration in mice, suggesting potential improvements for hair regeneration treatments in conditions like androgenetic alopecia. Our plain-language summary of this paper — not a Tressless recommendation.

The study developed a co-flowing microfluidic-assisted technology to produce dual aqueous microdroplets for hair regeneration. These microspheres, named G/HAD, were created using glycosaminoglycan-based photo-crosslinkable biological macromolecule (HAD) shells and gelatin methacrylate (GelMA) cores to enclose mesenchymal cells (MSCs) and mouse epidermal cells (EPCs). The G/HAD microspheres showed favorable characteristics, including sustained growth factor release and wet adhesion properties. After being transplanted into the dorsal skin of BALB/c nude mice, the G/HAD cell microspheres efficiently induced the regeneration of hair follicles. This method allows for the production of approximately 250 dual-layer microspheres per minute, showing potential in improving current hair regeneration techniques.

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