One-Step Generation of Core–Shell Biomimetic Microspheres Encapsulating Double-Layer Cells Using Microfluidics for Hair Regeneration

The study presents a microfluidic-assisted technology to create double-layer cell microspheres for hair regeneration. These microspheres encapsulate mouse mesenchymal cells and epidermal cells within gelatin methacrylate cores and photo-curable catechol-grafted hyaluronic acid shells. The resulting GelMA-MSC/HAD-EPC microspheres demonstrate rapid gelation, phase separation, biocompatibility, and wet adhesion, supporting cell proliferation and sustained growth factor release. When transplanted into the dorsal dermis of nude mice, these microspheres efficiently generate hair follicles. This method allows for the large-scale production of approximately 80 double-layer cell spheres per minute, offering a promising strategy for enhancing hair-regenerative medicine and potentially other organoid applications.